Design and characterization of a chitosan physical gel promoting wound healing in mice

Laura Mayol; Daniela De Stefano; Virginia Campani; Francesca De Falco; Eleonora Ferrari; Claudia Cencetti; Pietro Matricardi; Luigi Maiuri; Rosa Carnuccio; Angela Gallo; Maria Chiara Maiuri; Giuseppe De Rosa

doi:10.1007/s10856-014-5175-7

Design and characterization of a chitosan physical gel promoting wound healing in mice

Laura Mayol, Daniela De Stefano, Virginia Campani, Francesca De Falco, Eleonora Ferrari, Claudia Cencetti, Pietro Matricardi, Luigi Maiuri, Rosa Carnuccio, Angela Gallo, Maria Chiara Maiuri, Giuseppe De Rosa

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.

Lingua originale	Inglese
pagine (da-a)	1483-1493
Numero di pagine	11
Rivista	Journal of Materials Science: Materials in Medicine
Volume	25
Numero di pubblicazione	6
DOI	https://doi.org/10.1007/s10856-014-5175-7
Stato di pubblicazione	Pubblicato - giu 2014
Pubblicato esternamente	Sì

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10.1007/s10856-014-5175-7

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Mayol, L., De Stefano, D., Campani, V., De Falco, F., Ferrari, E., Cencetti, C., Matricardi, P., Maiuri, L., Carnuccio, R., Gallo, A., Maiuri, M. C., & De Rosa, G. (2014). Design and characterization of a chitosan physical gel promoting wound healing in mice. Journal of Materials Science: Materials in Medicine, 25(6), 1483-1493. https://doi.org/10.1007/s10856-014-5175-7

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title = "Design and characterization of a chitosan physical gel promoting wound healing in mice",

abstract = "In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.",

author = "Laura Mayol and {De Stefano}, Daniela and Virginia Campani and {De Falco}, Francesca and Eleonora Ferrari and Claudia Cencetti and Pietro Matricardi and Luigi Maiuri and Rosa Carnuccio and Angela Gallo and Maiuri, {Maria Chiara} and {De Rosa}, Giuseppe",

note = "Funding Information: Acknowledgments This work was supported by Italian {\textquoteleft}{\textquoteleft}Ministero della Salute{\textquoteright}{\textquoteright} (Grant: Progetto Giovani Ricercatori – annualit{\`a} 2007). The authors thank Dr. Manuela Gavina for her technical support.",

year = "2014",

month = jun,

doi = "10.1007/s10856-014-5175-7",

language = "English",

volume = "25",

pages = "1483--1493",

journal = "Journal of Materials Science: Materials in Medicine",

issn = "0957-4530",

publisher = "Springer Netherlands",

number = "6",

}

Mayol, L, De Stefano, D, Campani, V, De Falco, F, Ferrari, E, Cencetti, C, Matricardi, P, Maiuri, L, Carnuccio, R, Gallo, A, Maiuri, MC & De Rosa, G 2014, 'Design and characterization of a chitosan physical gel promoting wound healing in mice', Journal of Materials Science: Materials in Medicine, vol. 25, n. 6, pagg. 1483-1493. https://doi.org/10.1007/s10856-014-5175-7

TY - JOUR

T1 - Design and characterization of a chitosan physical gel promoting wound healing in mice

AU - Mayol, Laura

AU - De Stefano, Daniela

AU - Campani, Virginia

AU - De Falco, Francesca

AU - Ferrari, Eleonora

AU - Cencetti, Claudia

AU - Matricardi, Pietro

AU - Maiuri, Luigi

AU - Carnuccio, Rosa

AU - Gallo, Angela

AU - Maiuri, Maria Chiara

AU - De Rosa, Giuseppe

N1 - Funding Information: Acknowledgments This work was supported by Italian ‘‘Ministero della Salute’’ (Grant: Progetto Giovani Ricercatori – annualità 2007). The authors thank Dr. Manuela Gavina for her technical support.

PY - 2014/6

Y1 - 2014/6

N2 - In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.

AB - In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.

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DO - 10.1007/s10856-014-5175-7

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SN - 0957-4530

VL - 25

SP - 1483

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JO - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

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ER -

Design and characterization of a chitosan physical gel promoting wound healing in mice

Abstract

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