TY - JOUR
T1 - Comparative transcriptome profiles of near-isogenic hexaploid wheat lines differing for effective alleles at the 2DL FHB resistance QTL
AU - Biselli, Chiara
AU - Bagnaresi, Paolo
AU - Faccioli, Primetta
AU - Hu, Xinkun
AU - Balcerzak, Margaret
AU - Mattera, Maria G.
AU - Yan, Zehong
AU - Ouellet, Therese
AU - Cattivelli, Luigi
AU - Valè, Giampiero
N1 - Publisher Copyright:
© 2018 Biselli, Bagnaresi, Faccioli, Hu, Balcerzak, Mattera, Yan, Ouellet, Cattivelli and Valè.
PY - 2018/1/30
Y1 - 2018/1/30
N2 - Fusarium head blight (FHB), caused by the fungus Fusarium graminearum, represents one of the major wheat diseases worldwide, determining severe yield losses and reduction of grain quality due to the accumulation of mycotoxins. The molecular response associated with the wheat 2DL FHBresistance QTL was mined through a comprehensive transcriptomic analysis of the early response to F. graminearum infection, at 3 days post-inoculation, in spikelets and rachis. The analyses were conducted on two near isogenic lines (NILs) differing for the presence of the 2DL QTL (2-2618, resistant 2DL+ and 2-2890, susceptible null). The general response to fungal infection in terms of mRNAs accumulation trend was similar in both NILs, even though involving an higher number of DEGs in the susceptible NIL, and included down-regulation of the primary and energy metabolism, up-regulation of enzymes implicated in lignin and phenylpropanoid biosynthesis, activation of hormons biosynthesis and signal transduction pathways and genes involved in redox homeostasis and transcriptional regulation. The search for candidate genes with expression profiles associated with the 2DL QTL for FHBresistance led to the discovery of processes differentially modulated in the R and S NILs related to cell wall metabolism, sugar and JA signaling, signal reception and transduction, regulation of the redox status and transcription factors. Wheat FHB response-related miRNAs differentially regulated were also identified as putatively implicated in the superoxide dismutase activities and affecting genes regulating responses to biotic/abiotic stresses and auxin signaling. Altered gene expression was also observed for fungal non-codingRNAs. The putative targets of two of these were represented by the wheat gene WIR1A, involvedin resistance response, anda gene encoding a jacalin-relatedlectin protein, which participate in biotic and abiotic stress response, supporting the presence of a cross-talk between the plant and the fungus.
AB - Fusarium head blight (FHB), caused by the fungus Fusarium graminearum, represents one of the major wheat diseases worldwide, determining severe yield losses and reduction of grain quality due to the accumulation of mycotoxins. The molecular response associated with the wheat 2DL FHBresistance QTL was mined through a comprehensive transcriptomic analysis of the early response to F. graminearum infection, at 3 days post-inoculation, in spikelets and rachis. The analyses were conducted on two near isogenic lines (NILs) differing for the presence of the 2DL QTL (2-2618, resistant 2DL+ and 2-2890, susceptible null). The general response to fungal infection in terms of mRNAs accumulation trend was similar in both NILs, even though involving an higher number of DEGs in the susceptible NIL, and included down-regulation of the primary and energy metabolism, up-regulation of enzymes implicated in lignin and phenylpropanoid biosynthesis, activation of hormons biosynthesis and signal transduction pathways and genes involved in redox homeostasis and transcriptional regulation. The search for candidate genes with expression profiles associated with the 2DL QTL for FHBresistance led to the discovery of processes differentially modulated in the R and S NILs related to cell wall metabolism, sugar and JA signaling, signal reception and transduction, regulation of the redox status and transcription factors. Wheat FHB response-related miRNAs differentially regulated were also identified as putatively implicated in the superoxide dismutase activities and affecting genes regulating responses to biotic/abiotic stresses and auxin signaling. Altered gene expression was also observed for fungal non-codingRNAs. The putative targets of two of these were represented by the wheat gene WIR1A, involvedin resistance response, anda gene encoding a jacalin-relatedlectin protein, which participate in biotic and abiotic stress response, supporting the presence of a cross-talk between the plant and the fungus.
KW - Disease resistance
KW - Fusarium head blight
KW - MiRNA-Seq
KW - Near isogenic lines
KW - RNA-Seq
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=85043364915&partnerID=8YFLogxK
U2 - 10.3389/fpls.2018.00037
DO - 10.3389/fpls.2018.00037
M3 - Article
SN - 1664-462X
VL - 9
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 37
ER -