TY - JOUR
T1 - Communication circulating platelet-derived extracellular vesicles are a hallmark of sars-cov-2 infection
AU - Cappellano, Giuseppe
AU - Raineri, Davide
AU - Rolla, Roberta
AU - Giordano, Mara
AU - Puricelli, Chiara
AU - Vilardo, Beatrice
AU - Manfredi, Marcello
AU - Cantaluppi, Vincenzo
AU - Sainaghi, Pier Paolo
AU - Castello, Luigi
AU - De Vita, Nello
AU - Scotti, Lorenza
AU - Vaschetto, Rosanna
AU - Dianzani, Umberto
AU - Chiocchetti, Annalisa
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/1
Y1 - 2021/1
N2 - Sars-Cov-2 infection causes fever and cough that may rapidly lead to acute respiratory distress syndrome (ARDS). Few biomarkers have been identified but, unfortunately, these are individually poorly specific, and novel biomarkers are needed to better predict patient outcome. The aim of this study was to evaluate the diagnostic performance of circulating platelets (PLT)-derived extracellular vesicles (EVs) as biomarkers for Sars-Cov-2 infection, by setting a rapid and reliable test on unmanipulated blood samples. PLT-EVs were quantified by flow cytometry on two independent cohorts of Sars-CoV-2+ (n = 69), Sars-Cov-2− (n = 62) hospitalized patients, and healthy controls. Diagnostic performance of PLT-EVs was evaluated by receiver operating characteristic (ROC) curve. PLT-EVs count were higher in Sars-Cov-2+ compared to Sars-Cov-2− patients or HC. ROC analysis of the combined cohorts showed an AUC = 0.79 and an optimal cut-off value of 1472 EVs/µL, with 75% sensitivity and 74% specificity. These data suggest that PLT-EVs might be an interesting biomarker deserving further investigations to test their predictive power.
AB - Sars-Cov-2 infection causes fever and cough that may rapidly lead to acute respiratory distress syndrome (ARDS). Few biomarkers have been identified but, unfortunately, these are individually poorly specific, and novel biomarkers are needed to better predict patient outcome. The aim of this study was to evaluate the diagnostic performance of circulating platelets (PLT)-derived extracellular vesicles (EVs) as biomarkers for Sars-Cov-2 infection, by setting a rapid and reliable test on unmanipulated blood samples. PLT-EVs were quantified by flow cytometry on two independent cohorts of Sars-CoV-2+ (n = 69), Sars-Cov-2− (n = 62) hospitalized patients, and healthy controls. Diagnostic performance of PLT-EVs was evaluated by receiver operating characteristic (ROC) curve. PLT-EVs count were higher in Sars-Cov-2+ compared to Sars-Cov-2− patients or HC. ROC analysis of the combined cohorts showed an AUC = 0.79 and an optimal cut-off value of 1472 EVs/µL, with 75% sensitivity and 74% specificity. These data suggest that PLT-EVs might be an interesting biomarker deserving further investigations to test their predictive power.
KW - COVID-19
KW - Circulating biomarker
KW - Extracellular vesicles
KW - Platelets
KW - Sars-Cov-2
UR - http://www.scopus.com/inward/record.url?scp=85099891001&partnerID=8YFLogxK
U2 - 10.3390/cells10010085
DO - 10.3390/cells10010085
M3 - Article
SN - 2073-4409
VL - 10
SP - 1
EP - 10
JO - Cells
JF - Cells
IS - 1
M1 - 85
ER -