Cloning and sequencing of a full length cDNA coding for human retinol-binding protein

V. Colantuoni; V. Romano; G. Bensi; C. Santoro; F. Costanzo; G. Raugei; R. Cortese

doi:10.1093/nar/11.22.7769

Cloning and sequencing of a full length cDNA coding for human retinol-binding protein

V. Colantuoni
, V. Romano
, G. Bensi
, C. Santoro
, F. Costanzo
, G. Raugei
, R. Cortese

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that the gene is present in one or few copies per haploid genome and is transcribed in a single mRNA species.

Lingua originale	Inglese
pagine (da-a)	7769-7776
Numero di pagine	8
Rivista	Nucleic Acids Research
Volume	11
Numero di pubblicazione	22
DOI	https://doi.org/10.1093/nar/11.22.7769
Stato di pubblicazione	Pubblicato - 25 nov 1983
Pubblicato esternamente	Sì

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10.1093/nar/11.22.7769

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title = "Cloning and sequencing of a full length cDNA coding for human retinol-binding protein",

abstract = "We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that the gene is present in one or few copies per haploid genome and is transcribed in a single mRNA species.",

author = "V. Colantuoni and V. Romano and G. Bensi and C. Santoro and F. Costanzo and G. Raugei and R. Cortese",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Anne Walter for typing this manuscript. V.C. was supported by a long-term EMBO fellowship, G.R. and G.B. have a grant from Ente Farmacolo-gico Italiano (EFI), Naples, Italy, and F.C. is the recipient of a training contract GBI-O47-D from the {"}Biomolecular Engineering Programme{"} of the Commission of the European Communities. The work performed in Italy was supported by a grant from CNR, Progetto finalizzato Ingegneria Genetica e basi molecolari delle malattie ereditarie.",

year = "1983",

month = nov,

day = "25",

doi = "10.1093/nar/11.22.7769",

language = "English",

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journal = "Nucleic Acids Research",

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TY - JOUR

T1 - Cloning and sequencing of a full length cDNA coding for human retinol-binding protein

AU - Colantuoni, V.

AU - Romano, V.

AU - Bensi, G.

AU - Santoro, C.

AU - Costanzo, F.

AU - Raugei, G.

AU - Cortese, R.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Anne Walter for typing this manuscript. V.C. was supported by a long-term EMBO fellowship, G.R. and G.B. have a grant from Ente Farmacolo-gico Italiano (EFI), Naples, Italy, and F.C. is the recipient of a training contract GBI-O47-D from the "Biomolecular Engineering Programme" of the Commission of the European Communities. The work performed in Italy was supported by a grant from CNR, Progetto finalizzato Ingegneria Genetica e basi molecolari delle malattie ereditarie.

PY - 1983/11/25

Y1 - 1983/11/25

N2 - We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that the gene is present in one or few copies per haploid genome and is transcribed in a single mRNA species.

AB - We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that the gene is present in one or few copies per haploid genome and is transcribed in a single mRNA species.

UR - https://www.scopus.com/pages/publications/0021112784

U2 - 10.1093/nar/11.22.7769

DO - 10.1093/nar/11.22.7769

M3 - Article

SN - 0305-1048

VL - 11

SP - 7769

EP - 7776

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 22

ER -

Cloning and sequencing of a full length cDNA coding for human retinol-binding protein

Abstract

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