TY - JOUR
T1 - Clonal differences in survival capacity, copper and zinc accumulation, and correlation with leaf polyamine levels in poplar
T2 - A large-scale field trial on heavily polluted soil
AU - Castiglione, S.
AU - Todeschini, V.
AU - Franchin, C.
AU - Torrigiani, P.
AU - Gastaldi, D.
AU - Cicatelli, A.
AU - Rinaudo, C.
AU - Berta, G.
AU - Biondi, S.
AU - Lingua, G.
N1 - Funding Information:
The authors wish to thank M. Sobrero, D. Vigani, and A. Peterson for technical help during plant growth and experiments, and the management and staff of KME Italy (especially M. Sterpi and B. Ciarlo) for assistance. The research was financed by the Italian Ministry of Environment, Land and Sea Protection within the project “Research and development in biotechnology applied to the protection of the environment” in collaboration with the Popular Republic of China to SC, by Associazione Ambiente-Territorio-Formazione to GB and GL, and by the Italian Ministry for Education, University and Research (PRIN 2003077418) to SB, SC and GL.
PY - 2009/7
Y1 - 2009/7
N2 - Three ex situ collections of poplar clones from natural populations of Populus alba and P. nigra growing in northern Italy were assessed for their genetic dissimilarity (GD) by means of amplified fragment length polymorphism (AFLP). The high GD evidenced within populations was exploited for screening 168 clones in a field trial on heavy metal-polluted soil. After one growth season, clonal differences in plant survival and growth were observed. On the basis of performance, six clones were singled out, and used to evaluate copper and zinc accumulation in different organs. Clonal differences in metal concentrations were most evident for leaves and stems; one clone of P. alba (AL35) had a distinctly higher concentration of both metals in the roots. Leaf polyamine (putrescine, spermidine, spermine) profiles correlated with tissue metal concentrations, depending on the clone, plant organ and metal. In particular, the high metal-accumulating clone AL35 exhibited a dramatically higher concentration of free and conjugated putrescine. Overall, the results indicate that, given the high GD of Populus even within populations, it is possible to identify genotypes best suited for soil clean-up, and useful also for investigating physiological markers associated with high metal accumulation/tolerance.
AB - Three ex situ collections of poplar clones from natural populations of Populus alba and P. nigra growing in northern Italy were assessed for their genetic dissimilarity (GD) by means of amplified fragment length polymorphism (AFLP). The high GD evidenced within populations was exploited for screening 168 clones in a field trial on heavy metal-polluted soil. After one growth season, clonal differences in plant survival and growth were observed. On the basis of performance, six clones were singled out, and used to evaluate copper and zinc accumulation in different organs. Clonal differences in metal concentrations were most evident for leaves and stems; one clone of P. alba (AL35) had a distinctly higher concentration of both metals in the roots. Leaf polyamine (putrescine, spermidine, spermine) profiles correlated with tissue metal concentrations, depending on the clone, plant organ and metal. In particular, the high metal-accumulating clone AL35 exhibited a dramatically higher concentration of free and conjugated putrescine. Overall, the results indicate that, given the high GD of Populus even within populations, it is possible to identify genotypes best suited for soil clean-up, and useful also for investigating physiological markers associated with high metal accumulation/tolerance.
KW - Copper
KW - Field trial
KW - Phytoremediation
KW - Polyamines
KW - Populus
KW - Zinc
UR - https://www.scopus.com/pages/publications/66949138427
U2 - 10.1016/j.envpol.2009.02.011
DO - 10.1016/j.envpol.2009.02.011
M3 - Article
SN - 0269-7491
VL - 157
SP - 2108
EP - 2117
JO - Environmental Pollution
JF - Environmental Pollution
IS - 7
ER -