TY - JOUR
T1 - Chronic alcohol abuse and endosseous implants
T2 - Linkage of in vitro osteoblast dysfunction to titanium osseointegration rate
AU - Torricelli, Paola
AU - Fini, Milena
AU - Giavaresi, Gianluca
AU - Rimondini, Lia
AU - Tschon, Matilde
AU - Rimondini, Roberto
AU - Carrassi, Antonio
AU - Giardino, Roberto
N1 - Funding Information:
Partly supported by funding from the Swedish Medical Research Council, the Karolinska Institute, and Ricerca Sanitaria Finalizzata Regione Piemonte.
PY - 2008/1/14
Y1 - 2008/1/14
N2 - Chronic alcohol consumption is associated with pathological effects on bone, and it is correlated with the increasing risk of osteoporosis and fractures. The negative effects of alcohol intake also influence bone repair processes and the osseointegration of implants. The aim of the present in vitro study was to investigate the proliferation and synthetic activity of osteoblasts isolated from the trabecular bone of rats previously exposed to 7-week intermittent exposure to ethanol vapour (EE-OB), and sham-aged rats (SA-OB), when cultured on standard commercially pure Ti (cpTi). Osteoblast proliferation (WST-1), alkaline phosphatase (ALP), osteocalcin (OC), collagen type I (CICP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and transforming growth factor-β1 (TGF-β1) were measured at 1, 7, and 14 days of culture. Our results showed a decrease in the cell viability and synthetic activity of osteoblasts exposed to ethanol when cultured on cpTi. Moreover, the release of local regulatory factors from osteoblasts was imbalanced: TGF-β1 production was reduced and TNF-α and IL-6 were up-regulated. These in vitro data suggest that alcohol abuse affects bone repair and decreases the ability to form bone around standard cpTi. Innovative surfaces and adjuvant therapies could be useful when implants are required in alcoholics.
AB - Chronic alcohol consumption is associated with pathological effects on bone, and it is correlated with the increasing risk of osteoporosis and fractures. The negative effects of alcohol intake also influence bone repair processes and the osseointegration of implants. The aim of the present in vitro study was to investigate the proliferation and synthetic activity of osteoblasts isolated from the trabecular bone of rats previously exposed to 7-week intermittent exposure to ethanol vapour (EE-OB), and sham-aged rats (SA-OB), when cultured on standard commercially pure Ti (cpTi). Osteoblast proliferation (WST-1), alkaline phosphatase (ALP), osteocalcin (OC), collagen type I (CICP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and transforming growth factor-β1 (TGF-β1) were measured at 1, 7, and 14 days of culture. Our results showed a decrease in the cell viability and synthetic activity of osteoblasts exposed to ethanol when cultured on cpTi. Moreover, the release of local regulatory factors from osteoblasts was imbalanced: TGF-β1 production was reduced and TNF-α and IL-6 were up-regulated. These in vitro data suggest that alcohol abuse affects bone repair and decreases the ability to form bone around standard cpTi. Innovative surfaces and adjuvant therapies could be useful when implants are required in alcoholics.
KW - Alcohol
KW - Biomaterials
KW - Osseointegration
KW - Osteoblasts
KW - Titanium
UR - http://www.scopus.com/inward/record.url?scp=37049037986&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2007.10.001
DO - 10.1016/j.tox.2007.10.001
M3 - Article
SN - 0300-483X
VL - 243
SP - 138
EP - 144
JO - Toxicology
JF - Toxicology
IS - 1-2
ER -