Chromatographic separation of a mixture of polynuclear aromatic hydrocarbons with gradient elution with gradient elution. Theoretical prediction of retention times from isocratic experimental data

An ealier mathematical model for the prediction of retention times, under gradient elution conditions, on the basis of experimental isocratic data was validated by examining different classes of chemical compounds (amines, aldehydes and hydroxy-, amino- and methylquinolines) with various stationary and mobile phases. Here, the range of validity of the model is extended by using several polynuclear aromatic hydrocarbons, which exhibit apolar properties and chemical geometries very different from those of the compounds previously envisaged. The present data and the earlier data are used to provide a definite relationship between the error of prediction and the retention times.