Chitin degradation by Synechococcus WH7803

Giovanna Capovilla; Kurt G. Castro; Silvio Collani; Sean M. Kearney; David M. Kehoe; Sallie W. Chisholm

doi:10.1038/s41598-023-47332-0

Chitin degradation by Synechococcus WH7803

Giovanna Capovilla
, Kurt G. Castro
, Silvio Collani
, Sean M. Kearney
, David M. Kehoe
, Sallie W. Chisholm

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

Lingua originale	Inglese
Numero di articolo	19944
Rivista	Scientific Reports
Volume	13
Numero di pubblicazione	1
DOI	https://doi.org/10.1038/s41598-023-47332-0
Stato di pubblicazione	Pubblicato - dic 2023
Pubblicato esternamente	Sì

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10.1038/s41598-023-47332-0

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abstract = "Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.",

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AU - Chisholm, Sallie W.

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AB - Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

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Chitin degradation by Synechococcus WH7803

Abstract

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