Chitin degradation by Synechococcus WH7803

Giovanna Capovilla; Kurt G. Castro; SILVIO COLLANI; Sean M. Kearney; David M. Kehoe; Sallie W. Chisholm

doi:10.1038/s41598-023-47332-0

Chitin degradation by Synechococcus WH7803

Giovanna Capovilla, Kurt G. Castro, SILVIO COLLANI, Sean M. Kearney, David M. Kehoe, Sallie W. Chisholm

Dipartimento per lo Sviluppo Sostenibile e la Transizione Ecologica

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

Lingua originale	Inglese
Rivista	Scientific Reports
Volume	13
Numero di pubblicazione	1
DOI	https://doi.org/10.1038/s41598-023-47332-0
Stato di pubblicazione	Pubblicato - 2023

Keywords

CRISPR
Synechococcus
chitin

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10.1038/s41598-023-47332-0

https://hdl.handle.net/11579/194706

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title = "Chitin degradation by Synechococcus WH7803",

abstract = "Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.",

keywords = "CRISPR, Synechococcus, chitin, CRISPR, Synechococcus, chitin",

author = "Giovanna Capovilla and Castro, {Kurt G.} and SILVIO COLLANI and Kearney, {Sean M.} and Kehoe, {David M.} and Chisholm, {Sallie W.}",

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AU - Castro, Kurt G.

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AU - Chisholm, Sallie W.

PY - 2023

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KW - chitin

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KW - chitin

UR - https://iris.uniupo.it/handle/11579/194706

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Chitin degradation by Synechococcus WH7803

Abstract

Keywords

OSS delle Nazioni Unite

Accesso al documento

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