TY - JOUR
T1 - Chemical and Biomolecular Characterization of Artemisia umbelliformis Lam., an Important Ingredient of the Alpine Liqueur "Genepì"
AU - Rubiolo, Patrizia
AU - Matteodo, Maura
AU - Bicchi, Carlo
AU - Appendino, Giovanni
AU - Gnavi, Giorgio
AU - Bertea, Cinzia
AU - Maffei, Massimo
PY - 2009/5/13
Y1 - 2009/5/13
N2 - Artemisia umbelliformis Lam., an important alpine plant used for the preparation of flavored beverages, showed a remarkable intraspecific variability, at both genomic and gene product (secondary metabolites) levels. The variability of A. umbelliformis Lam. currently cultivated in Piedmont (Italy, Au1) and in Switzerland (Au2) was investigated by combining the chemical analysis of essential oil and sesquiterpene lactones and the molecular characterization of the 5S-rRNA-NTS gene by PCR and PCRRFLP. Marked differences were observed between the two plants. Au1 essential oil contained R- and β-thujones as the main components, whereas Au2 contained 1,8-cineole, borneol, and β-pinene. Au1 sesquiterpene lactone fractions contained cis-8-eudesmanolide derivatives and Au2 the trans-6- germacranolide costunolide. Specific A. umbelliformis Au1 and Au2 primers were designed on the sequence of the 5S-rRNA gene spacer region. Furthermore, a PCR-restriction fragment length polymorphism (PCR-RFLP) method was applied using RsaI and TaqI restriction enzymes. Chemical and biomolecular data contributed to the characterization of A. umbeliformis chemotypes.
AB - Artemisia umbelliformis Lam., an important alpine plant used for the preparation of flavored beverages, showed a remarkable intraspecific variability, at both genomic and gene product (secondary metabolites) levels. The variability of A. umbelliformis Lam. currently cultivated in Piedmont (Italy, Au1) and in Switzerland (Au2) was investigated by combining the chemical analysis of essential oil and sesquiterpene lactones and the molecular characterization of the 5S-rRNA-NTS gene by PCR and PCRRFLP. Marked differences were observed between the two plants. Au1 essential oil contained R- and β-thujones as the main components, whereas Au2 contained 1,8-cineole, borneol, and β-pinene. Au1 sesquiterpene lactone fractions contained cis-8-eudesmanolide derivatives and Au2 the trans-6- germacranolide costunolide. Specific A. umbelliformis Au1 and Au2 primers were designed on the sequence of the 5S-rRNA gene spacer region. Furthermore, a PCR-restriction fragment length polymorphism (PCR-RFLP) method was applied using RsaI and TaqI restriction enzymes. Chemical and biomolecular data contributed to the characterization of A. umbeliformis chemotypes.
KW - Alpine liqueurs
KW - Artemisia umbelliformis Lam.
KW - Biomolecular analysis
KW - Chemical analysis
KW - Chemotype discrimination
UR - http://www.scopus.com/inward/record.url?scp=66149146757&partnerID=8YFLogxK
U2 - 10.1021/jf803915v
DO - 10.1021/jf803915v
M3 - Article
SN - 0021-8561
VL - 57
SP - 3436
EP - 3443
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 9
ER -