Chemical and Biomolecular Characterization of Artemisia umbelliformis Lam., an Important Ingredient of the Alpine Liqueur "Genepì"

Patrizia Rubiolo, Maura Matteodo, Carlo Bicchi, Giovanni Appendino, Giorgio Gnavi, Cinzia Bertea, Massimo Maffei

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Artemisia umbelliformis Lam., an important alpine plant used for the preparation of flavored beverages, showed a remarkable intraspecific variability, at both genomic and gene product (secondary metabolites) levels. The variability of A. umbelliformis Lam. currently cultivated in Piedmont (Italy, Au1) and in Switzerland (Au2) was investigated by combining the chemical analysis of essential oil and sesquiterpene lactones and the molecular characterization of the 5S-rRNA-NTS gene by PCR and PCRRFLP. Marked differences were observed between the two plants. Au1 essential oil contained R- and β-thujones as the main components, whereas Au2 contained 1,8-cineole, borneol, and β-pinene. Au1 sesquiterpene lactone fractions contained cis-8-eudesmanolide derivatives and Au2 the trans-6- germacranolide costunolide. Specific A. umbelliformis Au1 and Au2 primers were designed on the sequence of the 5S-rRNA gene spacer region. Furthermore, a PCR-restriction fragment length polymorphism (PCR-RFLP) method was applied using RsaI and TaqI restriction enzymes. Chemical and biomolecular data contributed to the characterization of A. umbeliformis chemotypes.

Lingua originaleInglese
pagine (da-a)3436-3443
Numero di pagine8
RivistaJournal of Agricultural and Food Chemistry
Volume57
Numero di pubblicazione9
DOI
Stato di pubblicazionePubblicato - 13 mag 2009
Pubblicato esternamente

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