Characterization of NAD uptake in mammalian cells

Richard A. Billington, Cristina Travelli, Emanuela Ercolano, Ubaldina Galli, Cintia Blasi Roman, Ambra A. Grolla, Pier Luigi Canonico, Fabrizio Condorelli, Armando A. Genazzani

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.

Lingua originaleInglese
pagine (da-a)6367-6374
Numero di pagine8
RivistaJournal of Biological Chemistry
Volume283
Numero di pubblicazione10
DOI
Stato di pubblicazionePubblicato - 7 mar 2008

Fingerprint

Entra nei temi di ricerca di 'Characterization of NAD uptake in mammalian cells'. Insieme formano una fingerprint unica.

Cita questo