Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

A. Gallina; F. Rossi; G. Milanesi; R. Rossi; A. Montecucco; G. Ciarrocchi

doi:10.1042/bj3120593

Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

A. Gallina, F. Rossi, G. Milanesi, R. Rossi, A. Montecucco, G. Ciarrocchi

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10⁸ Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

Lingua originale	Inglese
pagine (da-a)	593-597
Numero di pagine	5
Rivista	Biochemical Journal
Volume	312
Numero di pubblicazione	2
DOI	https://doi.org/10.1042/bj3120593
Stato di pubblicazione	Pubblicato - 1995
Pubblicato esternamente	Sì

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title = "Characterization of human DNA ligase I expressed in a baculovirus-insect cell system",

abstract = "The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein",

author = "A. Gallina and F. Rossi and G. Milanesi and R. Rossi and A. Montecucco and G. Ciarrocchi",

year = "1995",

doi = "10.1042/bj3120593",

language = "English",

volume = "312",

pages = "593--597",

journal = "Biochemical Journal",

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T1 - Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

AU - Gallina, A.

AU - Rossi, F.

AU - Milanesi, G.

AU - Rossi, R.

AU - Montecucco, A.

AU - Ciarrocchi, G.

PY - 1995

Y1 - 1995

N2 - The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

AB - The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 108 Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein

UR - https://www.scopus.com/pages/publications/0029609364

U2 - 10.1042/bj3120593

DO - 10.1042/bj3120593

M3 - Article

SN - 0264-6021

VL - 312

SP - 593

EP - 597

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

Abstract

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