TY - CHAP
T1 - Challenges in biocatalysis
T2 - immobilization of pepsin in mesoporous silicates
AU - Manyar, Haresh G.
AU - Tumbiolo, Simonetta
AU - Gianotti, Enrica
AU - Sakamoto, Yasuhiro
AU - Terasaki, Osamu
AU - Coluccia, Salvatore
PY - 2008
Y1 - 2008
N2 - A facile preparation of Silico-Pepsin biocatalysts within mesoporous silicas, MCM-41 and SBA-15 has been achieved, by encapsulation of the enzyme. The shrinking of the pores was obtained by grafting with 3-aminopropyltriethoxysilane (APTES). The hybrid bio-inorganic materials were characterized by XRD, TEM, Volumetric (N2 sorption), FT-IR and DR-UV-Vis techniques and their catalytic activity was evaluated by peptic hydrolysis of a dipeptide, Z-L-glutamyl-L-tyrosine solution.
AB - A facile preparation of Silico-Pepsin biocatalysts within mesoporous silicas, MCM-41 and SBA-15 has been achieved, by encapsulation of the enzyme. The shrinking of the pores was obtained by grafting with 3-aminopropyltriethoxysilane (APTES). The hybrid bio-inorganic materials were characterized by XRD, TEM, Volumetric (N2 sorption), FT-IR and DR-UV-Vis techniques and their catalytic activity was evaluated by peptic hydrolysis of a dipeptide, Z-L-glutamyl-L-tyrosine solution.
KW - MCM-41
KW - Pepsin
KW - SBA-15
KW - biocatalysts
KW - enzyme immobilization
KW - hybrid bio-inorganic materials
KW - mesoporous silicates
UR - https://www.scopus.com/pages/publications/55149116109
U2 - 10.1016/S0167-2991(08)80134-6
DO - 10.1016/S0167-2991(08)80134-6
M3 - Chapter
AN - SCOPUS:55149116109
SN - 9780444532985
T3 - Studies in Surface Science and Catalysis
SP - 1327
EP - 1330
BT - Zeolites and related materials
ER -