TY - JOUR
T1 - Carbon tetrachloride-induced inhibition of protein kinase C in isolated rat hepatocytes
AU - Poli, Giuseppe
AU - Albano, Emanuele
AU - Dianzani, Mario U.
AU - Melloni, Edon
AU - Pontremoli, Sandro
AU - Marinari, Umberto M.
AU - Pronzato, Maria Adelaide
AU - Cottalasso, Damiano
N1 - Funding Information:
This work has been supported by the Ministero Pubblica Istruzione (Progetto Cirrosi Epatica) and by the Consiglio Nazionale delle Ricerche, (Progetto Finalizzato Oncologia). The authors are also grateful to the International Association for Cancer Research, London, for finantial support.
PY - 1988/6/16
Y1 - 1988/6/16
N2 - Isolated rat hepatocytes exposed to CCl4 showed a dramatic decrease in [32P] incorporation into proteins which was evident as early as 5 min after the haloalkane addition. DEAE cellulose separation of protein kinases present in both particulated and cytosolic fractions of hepatocytes revealed that only the calcium and phospholipids dependent protein kinase C was affected by the treatment with CCl4, while kinases not requiring these factors for their activity were unmodified. Several 4-hydroxyunsaturated aldehydes known to be produced during CCl4-stimulated lipid peroxidation were found to inhibit protein kinase C at micromolar concentrations, suggesting the possibility that peroxidative events might be responsible for the impairment of protein kinase C during CCl4 intoxication.
AB - Isolated rat hepatocytes exposed to CCl4 showed a dramatic decrease in [32P] incorporation into proteins which was evident as early as 5 min after the haloalkane addition. DEAE cellulose separation of protein kinases present in both particulated and cytosolic fractions of hepatocytes revealed that only the calcium and phospholipids dependent protein kinase C was affected by the treatment with CCl4, while kinases not requiring these factors for their activity were unmodified. Several 4-hydroxyunsaturated aldehydes known to be produced during CCl4-stimulated lipid peroxidation were found to inhibit protein kinase C at micromolar concentrations, suggesting the possibility that peroxidative events might be responsible for the impairment of protein kinase C during CCl4 intoxication.
UR - http://www.scopus.com/inward/record.url?scp=0024289909&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81136-7
DO - 10.1016/S0006-291X(88)81136-7
M3 - Article
SN - 0006-291X
VL - 153
SP - 591
EP - 597
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -