@article{bb6ea89ed05e45729e5b001844ed99f7,
title = "Biological performances of collagen-based scaffolds for vascular tissue engineering",
abstract = "Collagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular tissue engineering. In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent haemocompatibility and cell viability performance assays. First, haemoglobin-free, thrombelastography and platelet adhesion tests were performed in order to investigate the blood contact performance. Secondly, specimens were seeded with endothelial cells and smooth muscle cells, and cell viability tests were carried out by MTT and SEM. Results show that neutralized acid-soluble type I collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation. Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells.",
keywords = "Blood compatibility, Collagen, Endothelial cells, Platelet adhesion, Smooth muscle cells",
author = "F. Boccafoschi and J. Habermehl and S. Vesentini and D. Mantovani",
note = "Funding Information: We would like to thank Chiara Arrigoni (Mario Negri Institute, Bergamo, Italy) and Marie-France C{\^o}t{\'e} (Bioengineering and Biotechnology Unit of the research center at the St. Francois d{\textquoteright}Assise Hospital, Quebec City, Canada) for their useful help and suggestions. We are grateful to Charles Doillon (Laval University Hospital Center, Quebec City, Canada) and Nicole Fortier (Haemostasis department, Laval University Hospital Center, Quebec City, Canada) for help and guidance. We would also like to thank all blood donors as well as the blood collection unit at St. Fran{\c c}ois d{\textquoteright}Assise Hospital (Quebec City, Canada). This work was supported by the Natural Science and Engineering Research Council of Canada, the Fonds Qu{\'e}becois de la Recherche sur les Natures et les Technologies, the NATO Science Program and the Quebec Ministry for International Relations.",
year = "2005",
month = dec,
doi = "10.1016/j.biomaterials.2005.05.052",
language = "English",
volume = "26",
pages = "7410--7417",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Ltd.",
number = "35",
}