Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Gian Carlo Avanzi; Alessandra Cessano; Maria Felice Brizzi; Steven C. Clark; Luigi Pegoraro; Lina Matera

doi:10.1016/0024-3205(89)90247-6

Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Gian Carlo Avanzi, Alessandra Cessano, Maria Felice Brizzi, Steven C. Clark, Luigi Pegoraro, Lina Matera

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

Lingua originale	Inglese
pagine (da-a)	2621-2626
Numero di pagine	6
Rivista	Life Sciences
Volume	45
Numero di pubblicazione	26
DOI	https://doi.org/10.1016/0024-3205(89)90247-6
Stato di pubblicazione	Pubblicato - 1989
Pubblicato esternamente	Sì

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title = "Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture",

abstract = "As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.",

author = "Avanzi, {Gian Carlo} and Alessandra Cessano and Brizzi, {Maria Felice} and Clark, {Steven C.} and Luigi Pegoraro and Lina Matera",

note = "Funding Information: This work was supported by CNR, Progetto and by Comitato Gigi Ghirotti.",

year = "1989",

doi = "10.1016/0024-3205(89)90247-6",

language = "English",

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T1 - Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

AU - Avanzi, Gian Carlo

AU - Cessano, Alessandra

AU - Brizzi, Maria Felice

AU - Clark, Steven C.

AU - Pegoraro, Luigi

AU - Matera, Lina

N1 - Funding Information: This work was supported by CNR, Progetto and by Comitato Gigi Ghirotti.

PY - 1989

Y1 - 1989

N2 - As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

AB - As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.

UR - http://www.scopus.com/inward/record.url?scp=0024791942&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(89)90247-6

DO - 10.1016/0024-3205(89)90247-6

M3 - Article

SN - 0024-3205

VL - 45

SP - 2621

EP - 2626

JO - Life Sciences

JF - Life Sciences

IS - 26

ER -

Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture

Abstract

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