Bioencapsulation of Oocytes and Granulosa Cells

Massimo Faustini; Giulio Curone; MARIA LUISA TORRE; Daniele Vigo

doi:10.1007/978-1-4939-8600-2_9

Bioencapsulation of Oocytes and Granulosa Cells

Massimo Faustini, Giulio Curone, MARIA LUISA TORRE, Daniele Vigo

Dipartimento di Scienze del Farmaco

Risultato della ricerca: Capitolo in libro/report/atti di convegno › Contributo in volume (Capitolo o Saggio) › peer review

Abstract

A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.

Lingua originale	Inglese
Titolo della pubblicazione ospite	Epithelial Cell Culture. Methods in Molecular Biology
Pagine	89-93
Numero di pagine	5
Volume	1817
DOI	https://doi.org/10.1007/978-1-4939-8600-2_9
Stato di pubblicazione	Pubblicato - 2018

Keywords

3D culture
Alginate
Encapsulation
Granulosa cells

Accesso al documento

10.1007/978-1-4939-8600-2_9

http://hdl.handle.net/11579/144388

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abstract = "A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.",

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AU - TORRE, MARIA LUISA

AU - Vigo, Daniele

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KW - Encapsulation

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KW - Alginate

KW - Encapsulation

KW - Granulosa cells

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BT - Epithelial Cell Culture. Methods in Molecular Biology

ER -

Bioencapsulation of Oocytes and Granulosa Cells

Abstract

Keywords

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