TY - JOUR
T1 - Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro
AU - Vernè, E.
AU - Ferraris, S.
AU - Vitale-Brovarone, C.
AU - Cochis, A.
AU - Rimondini, L.
PY - 2014/9/15
Y1 - 2014/9/15
N2 - Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.
AB - Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability.
KW - Alkaline phosphatase
KW - Bioactive glasses
KW - Osteoblasts
KW - Surface functionalization
UR - http://www.scopus.com/inward/record.url?scp=84904817357&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2014.06.001
DO - 10.1016/j.apsusc.2014.06.001
M3 - Article
SN - 0169-4332
VL - 313
SP - 372
EP - 381
JO - Applied Surface Science
JF - Applied Surface Science
ER -