TY - JOUR
T1 - Bifunctional mesoporous glasses for bone tissue engineering
T2 - Biological effects of doping with cerium and polyphenols in 2D and 3D in vitro models
AU - Menshikh, Ksenia
AU - Reddy, Ajay Kumar
AU - Cochis, Andrea
AU - Fraulini, Francesca
AU - Zambon, Alfonso
AU - Lusvardi, Gigliola
AU - Rimondini, Lia
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/6
Y1 - 2024/6
N2 - This study evaluates the cytocompatibility of cerium-doped mesoporous bioactive glasses (Ce-MBGs) loaded with polyphenols (Ce-MBGs-Poly) for possible application in bone tissue engineering after tumour resection. We tested MBGs powders and pellets on 2D and 3D in vitro models using human bone marrow-derived mesenchymal stem cells (hMSCs), osteosarcoma cells (U2OS), and endothelial cells (EA.hy926). Promisingly, at a low concentration in culture medium, Poly-loaded MBGs powders containing 1.2 mol% of cerium inhibited U2OS metabolic activity, preserved hMSCs viability, and had no adverse effects on EA.hy926 migration. Moreover, the study discussed the possible interaction between cerium and Poly, influencing anti-cancer effects. In summary, this research provides insights into the complex interactions between Ce-MBGs, Poly, and various cell types in distinct 2D and 3D in vitro models, highlighting the potential of loaded Ce-MBGs for post-resection bone tissue engineering with a balance between pro-regenerative and anti-tumorigenic activities.
AB - This study evaluates the cytocompatibility of cerium-doped mesoporous bioactive glasses (Ce-MBGs) loaded with polyphenols (Ce-MBGs-Poly) for possible application in bone tissue engineering after tumour resection. We tested MBGs powders and pellets on 2D and 3D in vitro models using human bone marrow-derived mesenchymal stem cells (hMSCs), osteosarcoma cells (U2OS), and endothelial cells (EA.hy926). Promisingly, at a low concentration in culture medium, Poly-loaded MBGs powders containing 1.2 mol% of cerium inhibited U2OS metabolic activity, preserved hMSCs viability, and had no adverse effects on EA.hy926 migration. Moreover, the study discussed the possible interaction between cerium and Poly, influencing anti-cancer effects. In summary, this research provides insights into the complex interactions between Ce-MBGs, Poly, and various cell types in distinct 2D and 3D in vitro models, highlighting the potential of loaded Ce-MBGs for post-resection bone tissue engineering with a balance between pro-regenerative and anti-tumorigenic activities.
KW - 3D in vitro models
KW - Cerium
KW - Cytocompatibility
KW - Mesoporous bioactive glasses
KW - Polyphenols
UR - http://www.scopus.com/inward/record.url?scp=85195396038&partnerID=8YFLogxK
U2 - 10.1016/j.bbiosy.2024.100095
DO - 10.1016/j.bbiosy.2024.100095
M3 - Article
SN - 2666-5344
VL - 14
JO - Biomaterials and Biosystems
JF - Biomaterials and Biosystems
M1 - 100095
ER -