Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine

Ugo Chiuminatto; Fabio Gosetti; Paolo Dossetto; Eleonora Mazzucco; Davide Zampieri; Elisa Robotti; Maria Carla Gennaro; Emilio Marengo

doi:10.1021/ac100607v

Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine

Ugo Chiuminatto, Fabio Gosetti, Paolo Dossetto, Eleonora Mazzucco, Davide Zampieri, Elisa Robotti, Maria Carla Gennaro, Emilio Marengo

Dipartimento di Scienze e Innovazione Tecnologica

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The study deals with a fast (analysis times of around 11 min) simultaneous identification and quantification in human urine of 42 drugs (21 therapeutic and 21 of abuse), through an automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry (SPE UHPLC-MS/MS). In the method validation, particular attention was devoted to the matrix effect, through matrix-matched calibration in blank urine, suitably diluted. For all the abuse drugs investigated, the limit of quantitation (LOQ) values are lower than the legal threshold concentration levels, making the method suitable for routine control. The whole procedure was applied in the analysis of urine of patients positive to the I level screening test.

Lingua originale	Inglese
pagine (da-a)	5636-5645
Numero di pagine	10
Rivista	Analytical Chemistry
Volume	82
Numero di pubblicazione	13
DOI	https://doi.org/10.1021/ac100607v
Stato di pubblicazione	Pubblicato - 1 lug 2010

Accesso al documento

10.1021/ac100607v

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Chiuminatto, U., Gosetti, F., Dossetto, P., Mazzucco, E., Zampieri, D., Robotti, E., Gennaro, M. C., & Marengo, E. (2010). Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine. Analytical Chemistry, 82(13), 5636-5645. https://doi.org/10.1021/ac100607v

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title = "Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine",

abstract = "The study deals with a fast (analysis times of around 11 min) simultaneous identification and quantification in human urine of 42 drugs (21 therapeutic and 21 of abuse), through an automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry (SPE UHPLC-MS/MS). In the method validation, particular attention was devoted to the matrix effect, through matrix-matched calibration in blank urine, suitably diluted. For all the abuse drugs investigated, the limit of quantitation (LOQ) values are lower than the legal threshold concentration levels, making the method suitable for routine control. The whole procedure was applied in the analysis of urine of patients positive to the I level screening test.",

author = "Ugo Chiuminatto and Fabio Gosetti and Paolo Dossetto and Eleonora Mazzucco and Davide Zampieri and Elisa Robotti and Gennaro, \{Maria Carla\} and Emilio Marengo",

year = "2010",

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doi = "10.1021/ac100607v",

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Chiuminatto, U, Gosetti, F, Dossetto, P, Mazzucco, E, Zampieri, D, Robotti, E, Gennaro, MC & Marengo, E 2010, 'Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine', Analytical Chemistry, vol. 82, n. 13, pagg. 5636-5645. https://doi.org/10.1021/ac100607v

Automated online solid phase extraction ultra high performance liquid chromatography method coupled with tandem mass spectrometry for determination of forty-two therapeutic drugs and drugs of abuse in human urine. / Chiuminatto, Ugo; Gosetti, Fabio; Dossetto, Paolo et al.
In: Analytical Chemistry, Vol. 82, N. 13, 01.07.2010, pag. 5636-5645.

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

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AU - Mazzucco, Eleonora

AU - Zampieri, Davide

AU - Robotti, Elisa

AU - Gennaro, Maria Carla

AU - Marengo, Emilio

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