TY - GEN
T1 - Application of fuzzy logic principles to the classification of 2D-PAGE maps belonging to human pancreatic cancers treated with trichostatin-A
AU - Marengo, Emilio
AU - Robotti, Elisa
AU - Cecconi, Daniela
AU - Scarpa, Aldo
AU - Righetti, Pier Giorgio
PY - 2004
Y1 - 2004
N2 - 2D gel-electrophoresis is the most widespread technique applied in the study of cellular protein patterns. The complexity of this technique is reflected in the production of complex samples (2D gel maps) which are very difficult to compare. In this paper a method is proposed based on fuzzy logic principles. The method can be summarised in four steps: 1) digitalisation of the image; 2) fuzzyfication of the digitalised image in order to consider the natural variability of the applied technique; 3) comparison of the samples and calculation of a similarity matrix; 4) application of Multidimensional Scaling technique to the similarity matrix, in order to verify the presence of clusters of samples. The investigated dataset consists of IS samples belonging to two different cell lines (Paca 44 and T3M4) of control (untreated) and drug-treated pancreatic ductal carcinoma cells.
AB - 2D gel-electrophoresis is the most widespread technique applied in the study of cellular protein patterns. The complexity of this technique is reflected in the production of complex samples (2D gel maps) which are very difficult to compare. In this paper a method is proposed based on fuzzy logic principles. The method can be summarised in four steps: 1) digitalisation of the image; 2) fuzzyfication of the digitalised image in order to consider the natural variability of the applied technique; 3) comparison of the samples and calculation of a similarity matrix; 4) application of Multidimensional Scaling technique to the similarity matrix, in order to verify the presence of clusters of samples. The investigated dataset consists of IS samples belonging to two different cell lines (Paca 44 and T3M4) of control (untreated) and drug-treated pancreatic ductal carcinoma cells.
UR - http://www.scopus.com/inward/record.url?scp=11144298711&partnerID=8YFLogxK
U2 - 10.1109/FUZZY.2004.1375749
DO - 10.1109/FUZZY.2004.1375749
M3 - Conference contribution
AN - SCOPUS:11144298711
SN - 0780383532
T3 - IEEE International Conference on Fuzzy Systems
SP - 359
EP - 364
BT - 2004 IEEE International Conference on Fuzzy Systems - Proceedings
T2 - 2004 IEEE International Conference on Fuzzy Systems - Proceedings
Y2 - 25 July 2004 through 29 July 2004
ER -