TY - JOUR
T1 - Alveolar socket preservation technique
T2 - Effect of biomaterial on bone regenerative pattern
AU - Canullo, Luigi
AU - Pellegrini, Gaia
AU - Canciani, Elena
AU - Heinemann, Friedhelm
AU - Galliera, Emanuela
AU - Dellavia, Claudia
N1 - Publisher Copyright:
© 2015 Elsevier GmbH
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Background There is a lack of evidence in the literature on the correlation between histomorphometric findings and gene/protein expression markers for bone metabolism. Aim Evaluation of the histological features, changes in protein expression and gene activation for specific markers of bone metabolism following application of the alveolar ridge preservation technique with magnesium-enriched hydroxyapatite (MgHA). Materials and methods For each patient (n = 15), bone samples were harvested after tooth extraction and processed for immunohistochemical and gene expression analysis (T0). Then, all alveolar sockets were grafted with MgHA. After 4 months (T1), bone samples were harvested for histomorphometrical, immunohistochemical and gene expression analysis. Gene expression and protein expression were evaluated for: RANK, RANKL, OPG, IL-6, TNF-α. Results For all markers, gene expression increased, but not significantly, from T0 to T1. The mean RANKL/OPG ratio was 1.88 ± 1.24. Protein expression increased significantly (p < 0.05) for TNF-α, IL-6, RANK and RANKL. Histomorphometrical features at T1 were not significantly related to protein expression at T0. Conclusions After ridge preservation with MgHA, markers for bone catabolism were activated. No significant correlation was found between histomorphometrical features of the regenerated tissue and protein expression at baseline.
AB - Background There is a lack of evidence in the literature on the correlation between histomorphometric findings and gene/protein expression markers for bone metabolism. Aim Evaluation of the histological features, changes in protein expression and gene activation for specific markers of bone metabolism following application of the alveolar ridge preservation technique with magnesium-enriched hydroxyapatite (MgHA). Materials and methods For each patient (n = 15), bone samples were harvested after tooth extraction and processed for immunohistochemical and gene expression analysis (T0). Then, all alveolar sockets were grafted with MgHA. After 4 months (T1), bone samples were harvested for histomorphometrical, immunohistochemical and gene expression analysis. Gene expression and protein expression were evaluated for: RANK, RANKL, OPG, IL-6, TNF-α. Results For all markers, gene expression increased, but not significantly, from T0 to T1. The mean RANKL/OPG ratio was 1.88 ± 1.24. Protein expression increased significantly (p < 0.05) for TNF-α, IL-6, RANK and RANKL. Histomorphometrical features at T1 were not significantly related to protein expression at T0. Conclusions After ridge preservation with MgHA, markers for bone catabolism were activated. No significant correlation was found between histomorphometrical features of the regenerated tissue and protein expression at baseline.
KW - Alveolar socket preservation
KW - Bone regeneration
KW - IL-6
KW - Immunohistochemistry
KW - Magnesium-enriched hydroxyapatite
KW - RANK/RANKL/OPG
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=84984843607&partnerID=8YFLogxK
U2 - 10.1016/j.aanat.2015.05.007
DO - 10.1016/j.aanat.2015.05.007
M3 - Article
SN - 0940-9602
VL - 206
SP - 73
EP - 79
JO - Annals of Anatomy
JF - Annals of Anatomy
ER -