Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Raffaella Calligaris; Stefania Bottardi; Susanna Cogoi; Isabel Apezteguia; Claudio Santoro

doi:10.1073/pnas.92.25.11598

Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Raffaella Calligaris, Stefania Bottardi, Susanna Cogoi, Isabel Apezteguia, Claudio Santoro

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

Lingua originale	Inglese
pagine (da-a)	11598-11602
Numero di pagine	5
Rivista	Proceedings of the National Academy of Sciences of the United States of America
Volume	92
Numero di pubblicazione	25
DOI	https://doi.org/10.1073/pnas.92.25.11598
Stato di pubblicazione	Pubblicato - 5 dic 1995
Pubblicato esternamente	Sì

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10.1073/pnas.92.25.11598

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abstract = "GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.",

author = "Raffaella Calligaris and Stefania Bottardi and Susanna Cogoi and Isabel Apezteguia and Claudio Santoro",

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Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor. / Calligaris, Raffaella; Bottardi, Stefania; Cogoi, Susanna et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, N. 25, 05.12.1995, pag. 11598-11602.

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

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AU - Bottardi, Stefania

AU - Cogoi, Susanna

AU - Apezteguia, Isabel

AU - Santoro, Claudio

PY - 1995/12/5

Y1 - 1995/12/5

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AB - GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

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Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Abstract

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