TY - JOUR
T1 - Altered fibronectin mRNA splicing in skin fibroblasts from Ehlers-Danlos syndrome patients
T2 - In situ hybridization analysis
AU - Colombi, Marina
AU - Moro, Laura
AU - Zoppi, Nicoletta
AU - Ghinelli, Annalisa
AU - Barlati, Sergio
N1 - Funding Information:
We thank Prof. G. Cetta for EDS stains and Ms. M.R. Nava and R. Rodella for secretarial assistance. This work was supported by C.N.R. Target Projects "Biotechnologies and Bioinstrumentations" and "Genetic Engineering", A.I.R.C., M.P.I. 40% and E.C. Concerted Action on H.C.T.D. 1990-92.
PY - 1991/12
Y1 - 1991/12
N2 - The expression of fibronectin (FN) mRNA isoforms generated by alternative splicing of the EDA region was studied by dot-blot and in situ hybridization, using specific FN cDNA probes, in skin fibroblasts from controls and Ehlers-Danlos Syndrome (EDS) patients (types III, IV, VII and non classified). An Image Analysis program was used for the quantitative evaluation and comparison of FN mRNAs levels in the different cell strains. The in situ hybridization analysis showed that FN mRNAs are homogeneously expressed in all cells of each fibroblast strain analyzed. While in control fibroblasts about 70% of FN mRNA isoforms contain the EDA region (EDA+ FN mRNAs), in EDS fibroblasts this fraction is reduced up to about 30%. This indicates that in the EDS fibroblasts analyzed a deregulation of the alternative splicing processes acting at the EDA region takes place.
AB - The expression of fibronectin (FN) mRNA isoforms generated by alternative splicing of the EDA region was studied by dot-blot and in situ hybridization, using specific FN cDNA probes, in skin fibroblasts from controls and Ehlers-Danlos Syndrome (EDS) patients (types III, IV, VII and non classified). An Image Analysis program was used for the quantitative evaluation and comparison of FN mRNAs levels in the different cell strains. The in situ hybridization analysis showed that FN mRNAs are homogeneously expressed in all cells of each fibroblast strain analyzed. While in control fibroblasts about 70% of FN mRNA isoforms contain the EDA region (EDA+ FN mRNAs), in EDS fibroblasts this fraction is reduced up to about 30%. This indicates that in the EDS fibroblasts analyzed a deregulation of the alternative splicing processes acting at the EDA region takes place.
UR - https://www.scopus.com/pages/publications/0026335068
U2 - 10.1016/0309-1651(91)90091-V
DO - 10.1016/0309-1651(91)90091-V
M3 - Article
SN - 0309-1651
VL - 15
SP - 1195
EP - 1206
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 12
ER -