Allele frequencies for the PowerPlex™ 16 STR loci in an Albanian population sample from Northern Italy

C. Robino; S. Gino; C. Torre

doi:10.1520/jfs15086j

Allele frequencies for the PowerPlex^™ 16 STR loci in an Albanian population sample from Northern Italy

C. Robino
, S. Gino
, C. Torre

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

Lingua originale	Inglese
pagine (da-a)	998-999
Numero di pagine	2
Rivista	Journal of Forensic Sciences
Volume	46
Numero di pubblicazione	4
DOI	https://doi.org/10.1520/jfs15086j
Stato di pubblicazione	Pubblicato - 2001
Pubblicato esternamente	Sì

Accesso al documento

10.1520/jfs15086j

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title = "Allele frequencies for the PowerPlex{\texttrademark} 16 STR loci in an Albanian population sample from Northern Italy",

abstract = "In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint{\texttrademark} PowerPlex{\texttrademark} System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm",

keywords = "Albanians, CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, DNA typing, FGA, Forensic science, Italy, PENTA D, PENTA E, Polymerase chain reaction, Population genetics, Short tandem repeats, TH01, TPOX, VWA",

author = "C. Robino and S. Gino and C. Torre",

year = "2001",

doi = "10.1520/jfs15086j",

language = "English",

volume = "46",

pages = "998--999",

journal = "Journal of Forensic Sciences",

issn = "0022-1198",

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AU - Robino, C.

AU - Gino, S.

AU - Torre, C.

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Y1 - 2001

N2 - In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

AB - In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm

KW - Albanians

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KW - D18S51

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KW - D3S1358

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KW - D7S820

KW - D8S1179

KW - DNA typing

KW - FGA

KW - Forensic science

KW - Italy

KW - PENTA D

KW - PENTA E

KW - Polymerase chain reaction

KW - Population genetics

KW - Short tandem repeats

KW - TH01

KW - TPOX

KW - VWA

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