TY - JOUR
T1 - Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation
AU - Perteghella, Sara
AU - Gaviraghi, Alessandro
AU - Cenadelli, Silvia
AU - Bornaghi, Valeria
AU - Galli, Andrea
AU - Crivelli, Barbara
AU - Vigani, Barbara
AU - Vigo, Daniele
AU - Chlapanidas, Theodora
AU - Faustini, Massimo
AU - Torre, Maria Luisa
N1 - Publisher Copyright:
© 2017 The Korean Society of Veterinary Science.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability of buffalo spermatozoa after AI. In this study, for the first time, the use of alginate encapsulation and cryopreservation of buffalo spermatozoa is described, and the same procedure was performed with Holstein Friesian (Bos taurus) semen. Results obtained from in vitro analyses indicate that the encapsulation process does not have detrimental effects (compared to controls) on quality parameters (membrane integrity, progressive motility, path average velocity) in either species. Similarly, there were no detrimental effects after cryopreservation in either species. The fertilizing potential of encapsulated and cryopreserved semen was evaluated after AI in 25 buffalo and 113 bovine females. Pregnancy rates were not affected in either species. The results of this study show proof of concept for the use of frozen semen controlled-release devices in buffalo.
AB - The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability of buffalo spermatozoa after AI. In this study, for the first time, the use of alginate encapsulation and cryopreservation of buffalo spermatozoa is described, and the same procedure was performed with Holstein Friesian (Bos taurus) semen. Results obtained from in vitro analyses indicate that the encapsulation process does not have detrimental effects (compared to controls) on quality parameters (membrane integrity, progressive motility, path average velocity) in either species. Similarly, there were no detrimental effects after cryopreservation in either species. The fertilizing potential of encapsulated and cryopreserved semen was evaluated after AI in 25 buffalo and 113 bovine females. Pregnancy rates were not affected in either species. The results of this study show proof of concept for the use of frozen semen controlled-release devices in buffalo.
KW - Alginic acid
KW - Artificial insemination
KW - Buffaloes
KW - Cryopreservation
KW - Drug compounding encapsulation
UR - http://www.scopus.com/inward/record.url?scp=85015279790&partnerID=8YFLogxK
U2 - 10.4142/jvs.2017.18.1.81
DO - 10.4142/jvs.2017.18.1.81
M3 - Article
SN - 1229-845X
VL - 18
SP - 81
EP - 88
JO - Journal of Veterinary Science
JF - Journal of Veterinary Science
IS - 1
ER -