Affinity chromatography purification of erythrocyte membrane proteins after selective labeling with trinitrobenzene sodium sulfonate

Guido Tarone, Maria Prat, Paolo M. Comoglio

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

2,4,6-Trinitrobenzene sodium sulfonate may be used, under appropriate conditions, for specific surface labeling of erythrocyte plasma membranes. Trinitrophenylated ghost proteins are easily purified by reverse immunoadsorption using rabbit anti-dinitrophenyl antibodies covalently linked to Sepharose 4B. The advantages of a method that permits selective labeling of membrane molecules with a hapten and their purification by affinity chromatography are obvious. The possible applications of such a method in the investigation of the composition of plasma membranes are discussed.

Lingua originaleInglese
pagine (da-a)214-221
Numero di pagine8
RivistaBiochimica et Biophysica Acta - Biomembranes
Volume311
Numero di pubblicazione2
DOI
Stato di pubblicazionePubblicato - 22 giu 1973
Pubblicato esternamente

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