TY - JOUR
T1 - Activation of human immunodeficiency virus long terminal repeat by arachidonic acid
AU - Carini, Rita
AU - Leonarduzzi, Gabriella
AU - Camandola, Simonetta
AU - Musso, Tiziana
AU - Varesio, Luigi
AU - Baeuerle, Patrick A.
AU - Poli, Giuseppe
N1 - Funding Information:
This work was supported by grants from the Italian Ministry for University, Scientific and Technological Research; the Italian Ministry of Health VI AIDS Project; the National Research Council Targeted Project “ACRO”; and the Italian Association for Cancer Research.
PY - 1997
Y1 - 1997
N2 - Arachidonic acid is the precursor of highly reactive mediators, including prostaglandins and leukotrienes, and the most abundant n-6 polyunsaturated fatty acid in mammalian cell membranes. It is released from phospholipids upon many inflammatory stimuli. In this study, a chloramphenicol acyltransferase reporter gene, under control of the human immunodeficiency virus-1 long terminal repeat, was strongly induced upon treating human promonocytes with arachidonic acid. The n-3 fatty acid eicosapentenoic, found in abundance in fish oil, had no effect. HIV-1 long terminal repeat activation by arachidonic acid was suppressed by inhibitors of both lipoxygenase and cyclooxygenase pathways, suggesting that metabolites, rather than arachidonic acid itself, mediated the stimulatory effect. This is the first report linking HIV-1 expression to the metabolism of arachidonic acid.
AB - Arachidonic acid is the precursor of highly reactive mediators, including prostaglandins and leukotrienes, and the most abundant n-6 polyunsaturated fatty acid in mammalian cell membranes. It is released from phospholipids upon many inflammatory stimuli. In this study, a chloramphenicol acyltransferase reporter gene, under control of the human immunodeficiency virus-1 long terminal repeat, was strongly induced upon treating human promonocytes with arachidonic acid. The n-3 fatty acid eicosapentenoic, found in abundance in fish oil, had no effect. HIV-1 long terminal repeat activation by arachidonic acid was suppressed by inhibitors of both lipoxygenase and cyclooxygenase pathways, suggesting that metabolites, rather than arachidonic acid itself, mediated the stimulatory effect. This is the first report linking HIV-1 expression to the metabolism of arachidonic acid.
KW - 5-HETE
KW - AIDS
KW - Arachidonate oxidative metabolism
KW - Eicosapentenoic acid
KW - Free radicals
KW - HIV-1 LTR
KW - PGE 2
UR - http://www.scopus.com/inward/record.url?scp=0029955452&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(96)00291-2
DO - 10.1016/S0891-5849(96)00291-2
M3 - Article
SN - 0891-5849
VL - 22
SP - 195
EP - 199
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 1-2
ER -
