Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

E. Bertino; D. Farinasso; M. Cavaletto; A. Coscia; G. Prandi; M. G. Giuffrida; S. Costa; C. Fabris; A. Conti

Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

E. Bertino, D. Farinasso, M. Cavaletto, A. Coscia, G. Prandi, M. G. Giuffrida, S. Costa, C. Fabris, A. Conti

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

Lingua originale	Inglese
pagine (da-a)	15-19
Numero di pagine	5
Rivista	Acta Biomedica
Volume	68
Numero di pubblicazione	SUPPL. 1
Stato di pubblicazione	Pubblicato - 1997
Pubblicato esternamente	Sì

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title = "Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements",

abstract = "Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.",

keywords = "Bovine β-lactoglobuim, Cow's milk proteins allergy, Cross-reaction, ELISA, Human milk",

author = "E. Bertino and D. Farinasso and M. Cavaletto and A. Coscia and G. Prandi and Giuffrida, \{M. G.\} and S. Costa and C. Fabris and A. Conti",

year = "1997",

language = "English",

volume = "68",

pages = "15--19",

journal = "Acta Biomedica",

issn = "0392-4203",

publisher = "Mattioli1885",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

AU - Bertino, E.

AU - Farinasso, D.

AU - Cavaletto, M.

AU - Coscia, A.

AU - Prandi, G.

AU - Giuffrida, M. G.

AU - Costa, S.

AU - Fabris, C.

AU - Conti, A.

PY - 1997

Y1 - 1997

N2 - Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

AB - Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

KW - Bovine β-lactoglobuim

KW - Cow's milk proteins allergy

KW - Cross-reaction

KW - ELISA

KW - Human milk

UR - https://www.scopus.com/pages/publications/2442457001

M3 - Article

SN - 0392-4203

VL - 68

SP - 15

EP - 19

JO - Acta Biomedica

JF - Acta Biomedica

IS - SUPPL. 1

ER -

Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

Abstract

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