Absence in human milk of bovine β-lactoglobulin ingested by the mother. Unreliability of ELISA measurements

E. Bertino, D. Farinasso, M. Cavaletto, A. Coscia, G. Prandi, M. G. Giuffrida, S. Costa, C. Fabris, A. Conti

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Objectives. To analyze the presence of bovine β-LG in breast milk. Methods. Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of β-LG immuno-like proteins (β-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. Results. β-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, β-casein and α-lactalbumin, were identified as cross-reacting antigens. Conclusions. False-positive results in ELISA determinations of bovine β-LG In human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.

Lingua originaleInglese
pagine (da-a)15-19
Numero di pagine5
RivistaActa Biomedica
Volume68
Numero di pubblicazioneSUPPL. 1
Stato di pubblicazionePubblicato - 1997
Pubblicato esternamente

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