A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

Jia Shu Yang; Helge Gad; Stella Y. Lee; Alexander Mironov; Leiliang Zhang; Galina V. Beznoussenko; Carmen Valente; Gabriele Turacchio; Akua N. Bonsra; Guangwei Du; Gianluca Baldanzi; Andrea Graziani; Sylvain Bourgoin; Michael A. Frohman; Alberto Luini; Victor W. Hsu

doi:10.1038/ncb1774

A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

Jia Shu Yang, Helge Gad, Stella Y. Lee, Alexander Mironov, Leiliang Zhang, Galina V. Beznoussenko, Carmen Valente, Gabriele Turacchio, Akua N. Bonsra, Guangwei Du, Gianluca Baldanzi, Andrea Graziani, Sylvain Bourgoin, Michael A. Frohman, Alberto Luini, Victor W. Hsu

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

Lingua originale	Inglese
pagine (da-a)	1146-1153
Numero di pagine	8
Rivista	Nature Cell Biology
Volume	10
Numero di pubblicazione	10
DOI	https://doi.org/10.1038/ncb1774
Stato di pubblicazione	Pubblicato - 2008
Pubblicato esternamente	Sì

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10.1038/ncb1774

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Yang, J. S., Gad, H., Lee, S. Y., Mironov, A., Zhang, L., Beznoussenko, G. V., Valente, C., Turacchio, G., Bonsra, A. N., Du, G., Baldanzi, G., Graziani, A., Bourgoin, S., Frohman, M. A., Luini, A., & Hsu, V. W. (2008). A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. Nature Cell Biology, 10(10), 1146-1153. https://doi.org/10.1038/ncb1774

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title = "A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance",

abstract = "Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.",

author = "Yang, {Jia Shu} and Helge Gad and Lee, {Stella Y.} and Alexander Mironov and Leiliang Zhang and Beznoussenko, {Galina V.} and Carmen Valente and Gabriele Turacchio and Bonsra, {Akua N.} and Guangwei Du and Gianluca Baldanzi and Andrea Graziani and Sylvain Bourgoin and Frohman, {Michael A.} and Alberto Luini and Hsu, {Victor W.}",

note = "Funding Information: We thank Jian Li and Ming Bai for advice and discussions, and Richard Premont for technical assistance. This work was funded by grants from the NIH to V.W.H. (GM058615), M.A.F. (GM071520) and G.D. (GM071475), from Telethon (Italy), AIRC (Italy) to A.L. and A.M., and from the Canadian Institutes of Health Research (S.B.). H.G. was supported by a Marie Curie Fellowship.",

year = "2008",

doi = "10.1038/ncb1774",

language = "English",

volume = "10",

pages = "1146--1153",

journal = "Nature Cell Biology",

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T1 - A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

AU - Yang, Jia Shu

AU - Gad, Helge

AU - Lee, Stella Y.

AU - Mironov, Alexander

AU - Zhang, Leiliang

AU - Beznoussenko, Galina V.

AU - Valente, Carmen

AU - Turacchio, Gabriele

AU - Bonsra, Akua N.

AU - Du, Guangwei

AU - Baldanzi, Gianluca

AU - Graziani, Andrea

AU - Bourgoin, Sylvain

AU - Frohman, Michael A.

AU - Luini, Alberto

AU - Hsu, Victor W.

N1 - Funding Information: We thank Jian Li and Ming Bai for advice and discussions, and Richard Premont for technical assistance. This work was funded by grants from the NIH to V.W.H. (GM058615), M.A.F. (GM071520) and G.D. (GM071475), from Telethon (Italy), AIRC (Italy) to A.L. and A.M., and from the Canadian Institutes of Health Research (S.B.). H.G. was supported by a Marie Curie Fellowship.

PY - 2008

Y1 - 2008

N2 - Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

AB - Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

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A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

Abstract

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