A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

R. Accomando; A. Viarengo; R. Bordone; M. Taningher; L. Canesi; M. Orunesu

doi:10.1016/0020-711X(91)90194-R

A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

R. Accomando, A. Viarengo, R. Bordone, M. Taningher, L. Canesi, M. Orunesu

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

Lingua originale	Inglese
pagine (da-a)	227-229
Numero di pagine	3
Rivista	International Journal of Biochemistry
Volume	23
Numero di pubblicazione	2
DOI	https://doi.org/10.1016/0020-711X(91)90194-R
Stato di pubblicazione	Pubblicato - 1991
Pubblicato esternamente	Sì

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10.1016/0020-711X(91)90194-R

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title = "A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals",

abstract = "1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.",

author = "R. Accomando and A. Viarengo and R. Bordone and M. Taningher and L. Canesi and M. Orunesu",

year = "1991",

doi = "10.1016/0020-711X(91)90194-R",

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T1 - A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

AU - Accomando, R.

AU - Viarengo, A.

AU - Bordone, R.

AU - Taningher, M.

AU - Canesi, L.

AU - Orunesu, M.

PY - 1991

Y1 - 1991

N2 - 1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

AB - 1. 1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.

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U2 - 10.1016/0020-711X(91)90194-R

DO - 10.1016/0020-711X(91)90194-R

M3 - Article

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JO - International Journal of Biochemistry

JF - International Journal of Biochemistry

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A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals

Abstract

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