A Pulsed Electric Field (PEF) bench static system to study bacteria inactivation

Pietro Cortese, Giuseppe Dellacasa, Roberto Gemme, Sara Bonetta, Silvia Bonetta, Elisabetta Carraro, Francesca Motta, Marco Paganoni, Marco Pizzichemi

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Pulsed Electric Fields (PEF) technology is a promising non-thermal processing method for inactivation of microorganisms. A small PEF bench system able to treat a 0.4 ml static liquid volume has been built and tested at the laboratories of the Università del Piemonte Orientale in Alessandria, Italy. The technique used to produce the required fields consists of charging high voltage cables of various lengths and subsequently discharge them on a cylindrical cell. The pulse intensity can be adjusted to reach a maximum electric field in the cell of about 35 kV/cm and the pulse frequency can reach 10 Hz. We describe the PEF system in some detail and, as a benchmark of its performances, we report preliminary results obtained on Escherichia coli (ATCC 25922) at 109 Cfu/ml concentration suspended in a McIlvaine buffer (pH 7.2).

Lingua originaleInglese
pagine (da-a)162-164
Numero di pagine3
RivistaNuclear Physics B - Proceedings Supplements
Volume215
Numero di pubblicazione1
DOI
Stato di pubblicazionePubblicato - giu 2011

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