TY - JOUR
T1 - A bioprintable gellan gum/lignin hydrogel
T2 - a smart and sustainable route for cartilage regeneration
AU - Bonifacio, Maria A.
AU - Cometa, Stefania
AU - Cochis, Andrea
AU - Scalzone, Annachiara
AU - Gentile, Piergiorgio
AU - Scalia, Alessandro C.
AU - Rimondini, Lia
AU - Mastrorilli, Piero
AU - De Giglio, Elvira
N1 - Publisher Copyright:
© 2022
PY - 2022/9/1
Y1 - 2022/9/1
N2 - In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.
AB - In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.
KW - Bioink
KW - Gellan gum
KW - Lignin
UR - http://www.scopus.com/inward/record.url?scp=85133803020&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2022.07.002
DO - 10.1016/j.ijbiomac.2022.07.002
M3 - Article
SN - 0141-8130
VL - 216
SP - 336
EP - 346
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -