2D-DIGE and fluorescence image analysis

Elisa ROBOTTI; Emilio MARENGO

doi:10.1007/978-1-4939-7268-5_3

2D-DIGE and fluorescence image analysis

Elisa ROBOTTI, Emilio MARENGO

Dipartimento di Scienze e Innovazione Tecnologica

Risultato della ricerca: Capitolo in libro/report/atti di convegno › Contributo in volume (Capitolo o Saggio)

Abstract

2D-DIGE is still a very widespread technique in proteomics for the identification of panels of biomarkers, allowing to tackle with some important drawback of classical two-dimensional gel-electrophoresis. However, once 2D-gels are obtained, they must undergo a quite articulated multistep image analysis procedure before the final differential analysis via statistical mono- and multivariate methods. Here, the main steps of image analysis software are described and the most recent procedures reported in the literature are briefly presented.

Lingua originale	Inglese
Titolo della pubblicazione ospite	Methods in Molecular Biology
Editore	Humana Press Inc.
Pagine	25-39
Numero di pagine	15
Volume	1664
ISBN (stampa)	978-1-4939-7267-8
DOI	https://doi.org/10.1007/978-1-4939-7268-5_3
Stato di pubblicazione	Pubblicato - 2018

Keywords

DIGE
Differential analysis
Genetics
Image analysis
Image preprocessing
Molecular Biology
Spot detection and quantification
Spot matching
Warping

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10.1007/978-1-4939-7268-5_3

http://hdl.handle.net/11579/90443

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KW - Genetics

KW - Image analysis

KW - Image preprocessing

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KW - Spot matching

KW - Warping

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2D-DIGE and fluorescence image analysis

Abstract

Keywords

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