@inbook{80ab83c0e2f14da38531008b4b5b5832,
title = "2D-DIGE and fluorescence image analysis",
abstract = "2D-DIGE is still a very widespread technique in proteomics for the identification of panels of biomarkers, allowing to tackle with some important drawback of classical two-dimensional gel-electrophoresis. However, once 2D-gels are obtained, they must undergo a quite articulated multistep image analysis procedure before the final differential analysis via statistical mono- and multivariate methods. Here, the main steps of image analysis software are described and the most recent procedures reported in the literature are briefly presented.",
keywords = "DIGE, Differential analysis, Image analysis, Image preprocessing, Spot detection and quantification, Spot matching, Warping",
author = "Elisa Robotti and Emilio Marengo",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7268-5_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--39",
booktitle = "Methods in Molecular Biology",
}