The role of ph of the mobile-phase in ion-interaction RP-HPLC

M. C. Gennaro; D. Giacosa; C. Abrigo

doi:10.1080/10826079408013623

The role of ph of the mobile-phase in ion-interaction RP-HPLC

M. C. Gennaro
, D. Giacosa
, C. Abrigo

Research output: Contribution to journal › Article › peer-review

Abstract

The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pk_a values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

Original language	English
Pages (from-to)	4365-4380
Number of pages	16
Journal	Journal of Liquid Chromatography
Volume	17
Issue number	20
DOIs	https://doi.org/10.1080/10826079408013623
Publication status	Published - 1 Dec 1994
Externally published	Yes

Access to Document

10.1080/10826079408013623

Cite this

@article{27d00feb7b4b4a72bc2d32c16e71be8e,

title = "The role of ph of the mobile-phase in ion-interaction RP-HPLC",

abstract = "The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.",

author = "Gennaro, \{M. C.\} and D. Giacosa and C. Abrigo",

year = "1994",

month = dec,

day = "1",

doi = "10.1080/10826079408013623",

language = "English",

volume = "17",

pages = "4365--4380",

journal = "Journal of Liquid Chromatography",

issn = "0148-3919",

publisher = "Marcel Dekker Inc.",

number = "20",

}

TY - JOUR

T1 - The role of ph of the mobile-phase in ion-interaction RP-HPLC

AU - Gennaro, M. C.

AU - Giacosa, D.

AU - Abrigo, C.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

AB - The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

UR - https://www.scopus.com/pages/publications/0028626311

U2 - 10.1080/10826079408013623

DO - 10.1080/10826079408013623

M3 - Article

SN - 0148-3919

VL - 17

SP - 4365

EP - 4380

JO - Journal of Liquid Chromatography

JF - Journal of Liquid Chromatography

IS - 20

ER -

The role of ph of the mobile-phase in ion-interaction RP-HPLC

Abstract

Access to Document

Other files and links

Fingerprint

Cite this