The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD synthetase

R CANTONI; M BRANZONI; M LAB; Menico RIZZI; G. RICCARDI

The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD synthetase

R CANTONI
, M BRANZONI
, M LAB
, Menico RIZZI
, G. RICCARDI

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD⁺ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His₆-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD⁺ synthetase.

Original language	English
Pages (from-to)	3218-3221
Number of pages	4
Journal	Journal of Bacteriology
Volume	180
Publication status	Published - 1998

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title = "The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD synthetase",

abstract = "The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.",

author = "R CANTONI and M BRANZONI and M LAB and Menico RIZZI and G. RICCARDI",

year = "1998",

language = "English",

volume = "180",

pages = "3218--3221",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD synthetase

AU - CANTONI, R

AU - BRANZONI, M

AU - LAB, M

AU - RIZZI, Menico

AU - RICCARDI, G.

PY - 1998

Y1 - 1998

N2 - The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.

AB - The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.

UR - https://iris.uniupo.it/handle/11579/9296

M3 - Article

SN - 0021-9193

VL - 180

SP - 3218

EP - 3221

JO - Journal of Bacteriology

JF - Journal of Bacteriology

ER -

The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD synthetase

Abstract

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