The interaction of SiO2 nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx

Carla Distasi; Marianna Dionisi; Federico Alessandro Ruffinatti; Alessandra Gilardino; Roberta Bardini; Susanna Antoniotti; Federico Catalano; Eleonora Bassino; Luca Munaron; Gianmario Martra; Davide Lovisolo

doi:10.2217/nnm-2018-0256

The interaction of SiO₂ nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx

Carla Distasi
, Marianna Dionisi
, Federico Alessandro Ruffinatti
, Alessandra Gilardino
, Roberta Bardini
, Susanna Antoniotti
, Federico Catalano
, Eleonora Bassino
, Luca Munaron
, Gianmario Martra
, Davide Lovisolo

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To clarify the mechanisms of interaction between SiO₂ nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO₂ NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca²⁺]_i increase. Conclusion: These findings suggest that SiO₂ NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

Original language	English
Pages (from-to)	575-594
Number of pages	20
Journal	Nanomedicine
Volume	8
Issue number	1
DOIs	https://doi.org/10.2217/nnm-2018-0256
Publication status	Published - Mar 2019

Keywords

Cx channels
Panx channels
TRPV4 channels
calcium
free radicals
neuronal cells
patch clamp
silica nanoparticles
single channel

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title = "The interaction of SiO2 nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx",

abstract = "Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials \& methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.",

keywords = "Cx channels, Panx channels, TRPV4 channels, calcium, free radicals, neuronal cells, patch clamp, silica nanoparticles, single channel",

author = "Carla Distasi and Marianna Dionisi and Ruffinatti, \{Federico Alessandro\} and Alessandra Gilardino and Roberta Bardini and Susanna Antoniotti and Federico Catalano and Eleonora Bassino and Luca Munaron and Gianmario Martra and Davide Lovisolo",

note = "Publisher Copyright: {\textcopyright} 2019 Future Medicine Ltd.",

year = "2019",

month = mar,

doi = "10.2217/nnm-2018-0256",

language = "English",

volume = "8",

pages = "575--594",

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TY - JOUR

T1 - The interaction of SiO2 nanoparticles with the neuronal cell membrane

T2 - Activation of ionic channels and calcium influx

AU - Distasi, Carla

AU - Dionisi, Marianna

AU - Ruffinatti, Federico Alessandro

AU - Gilardino, Alessandra

AU - Bardini, Roberta

AU - Antoniotti, Susanna

AU - Catalano, Federico

AU - Bassino, Eleonora

AU - Munaron, Luca

AU - Martra, Gianmario

AU - Lovisolo, Davide

PY - 2019/3

Y1 - 2019/3

N2 - Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

AB - Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

KW - Cx channels

KW - Panx channels

KW - TRPV4 channels

KW - calcium

KW - free radicals

KW - neuronal cells

KW - patch clamp

KW - silica nanoparticles

KW - single channel

UR - https://www.scopus.com/pages/publications/85062849455

U2 - 10.2217/nnm-2018-0256

DO - 10.2217/nnm-2018-0256

M3 - Article

SN - 1743-5889

VL - 8

SP - 575

EP - 594

JO - Nanomedicine

JF - Nanomedicine

IS - 1

ER -

The interaction of SiO₂ nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx

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Keywords

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