The anti-apoptotic Bcl-xl protein, a new piece in the puzzle of cytochrome c interactome

Ivano Bertini; Soizic Chevance; Rebecca Del Conte; Daniela Lalli; Paola Turano

doi:10.1371/journal.pone.0018329

The anti-apoptotic Bcl-x_l protein, a new piece in the puzzle of cytochrome c interactome

Ivano Bertini, Soizic Chevance, Rebecca Del Conte, Daniela Lalli, Paola Turano

Research output: Contribution to journal › Article › peer-review

Abstract

A structural model of the adduct between human cytochrome c and the human anti-apoptotic protein Bcl-x_L, which defines the protein-protein interaction surface, was obtained from solution NMR chemical shift perturbation data. The atomic level information reveals key intermolecular contacts identifying new potentially druggable areas on cytochrome c and Bcl-x_L. Involvement of residues on cytochrome c other than those in its complexes with electron transfer partners is apparent. Key differences in the contact area also exist between the Bcl-x_L adduct with the Bak peptide and that with cytochrome c. The present model provides insights to the mechanism by which cytochrome c translocated to cytosol can be intercepted, so that the apoptosome is not assembled.

Original language	English
Article number	e18329
Journal	PLoS ONE
Volume	6
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0018329
Publication status	Published - 2011
Externally published	Yes

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abstract = "A structural model of the adduct between human cytochrome c and the human anti-apoptotic protein Bcl-xL, which defines the protein-protein interaction surface, was obtained from solution NMR chemical shift perturbation data. The atomic level information reveals key intermolecular contacts identifying new potentially druggable areas on cytochrome c and Bcl-xL. Involvement of residues on cytochrome c other than those in its complexes with electron transfer partners is apparent. Key differences in the contact area also exist between the Bcl-xL adduct with the Bak peptide and that with cytochrome c. The present model provides insights to the mechanism by which cytochrome c translocated to cytosol can be intercepted, so that the apoptosome is not assembled.",

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AU - Turano, Paola

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The anti-apoptotic Bcl-x_l protein, a new piece in the puzzle of cytochrome c interactome

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