The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro RAVERA; G BAGNI; M MASCINI; J. C DABROWIAK; Domenico OSELLA

doi:10.1016/j.jinorgbio.2007.03.017

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro RAVERA
, G BAGNI
, M MASCINI
, J. C DABROWIAK
, Domenico OSELLA

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

Original language	English
Pages (from-to)	1023-1027
Number of pages	5
Journal	Journal of Inorganic Biochemistry
Volume	101
DOIs	https://doi.org/10.1016/j.jinorgbio.2007.03.017
Publication status	Published - 2007

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10.1016/j.jinorgbio.2007.03.017

http://hdl.handle.net/11579/30139

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@article{6545cad3da1846c78e0a7b815567fcba,

title = "The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor",

abstract = "We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.",

author = "Mauro RAVERA and G BAGNI and M MASCINI and DABROWIAK, \{J. C\} and Domenico OSELLA",

note = "Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).",

year = "2007",

doi = "10.1016/j.jinorgbio.2007.03.017",

language = "English",

volume = "101",

pages = "1023--1027",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

AU - RAVERA, Mauro

AU - BAGNI, G

AU - MASCINI, M

AU - DABROWIAK, J. C

AU - OSELLA, Domenico

N1 - Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).

PY - 2007

Y1 - 2007

N2 - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

AB - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

UR - https://iris.uniupo.it/handle/11579/30139

U2 - 10.1016/j.jinorgbio.2007.03.017

DO - 10.1016/j.jinorgbio.2007.03.017

M3 - Article

SN - 0162-0134

VL - 101

SP - 1023

EP - 1027

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

ER -

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

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