Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique

Marcello Manfredi; Simona Martinotti; Mauro Patrone; Maria Paola Sassi; Elia Ranzato; Emilio Marengo

doi:10.1109/MeMeA.2015.7145260

Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique

Marcello Manfredi, Simona Martinotti, Mauro Patrone, Maria Paola Sassi, Elia Ranzato, Emilio Marengo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Mass spectrometry (MS)-based methods have become popular in recent years for the quantitation of biomolecules, especially peptides and proteins. High Mobility Group Box-1 protein (HMGB1) is a nuclear DNA-binding protein that resides inside the nucleus and can be released to the extracellular space under specific conditions. HMGB1 has been reported as circulating mechanistic indicators of cell death mode in animal models and in clinical studies. There is accumulating evidence that HMGB1 contributes to the pathogenesis of inflammatory and autoimmune diseases. Moreover, HMGB1 has been found to play an important role in tumor development, growth, and spread. Circulating HMGB1 level could be a useful and specific marker for evaluating different disease status and predicting prognosis in patients. The label-free quantitative comparison without any standard reference or normalization of the target peptide signal is the simplest Liquid Chromatographic-MS (LC-MS) method for the quantitation of a protein. The aim of the research was the development of an analytical method for the quantitation of HMGB1 by a label-free Mass Spectrometry technique. In this paper are presented the preliminary results: the quantitation of HMGB1 was assessed in a cell lysate sample through the use of the standard concentration curve of purified HMGB1 protein.

Original language	English
Title of host publication	2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	526-530
Number of pages	5
ISBN (Electronic)	9781479964765
DOIs	https://doi.org/10.1109/MeMeA.2015.7145260
Publication status	Published - 30 Jun 2015
Event	2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Torino, Italy Duration: 7 May 2015 → 9 May 2015

Publication series

Name	2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings

Conference

Conference	2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015
Country/Territory	Italy
City	Torino
Period	7/05/15 → 9/05/15

Keywords

HMGB1 quantification
Targeted proteomics
shotgun analysis

Access to Document

10.1109/MeMeA.2015.7145260

Cite this

Manfredi, M., Martinotti, S., Patrone, M., Sassi, M. P., Ranzato, E., & Marengo, E. (2015). Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique. In 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings (pp. 526-530). Article 7145260 (2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MeMeA.2015.7145260

Manfredi, Marcello ; Martinotti, Simona ; Patrone, Mauro et al. / Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique. 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 526-530 (2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings).

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abstract = "Mass spectrometry (MS)-based methods have become popular in recent years for the quantitation of biomolecules, especially peptides and proteins. High Mobility Group Box-1 protein (HMGB1) is a nuclear DNA-binding protein that resides inside the nucleus and can be released to the extracellular space under specific conditions. HMGB1 has been reported as circulating mechanistic indicators of cell death mode in animal models and in clinical studies. There is accumulating evidence that HMGB1 contributes to the pathogenesis of inflammatory and autoimmune diseases. Moreover, HMGB1 has been found to play an important role in tumor development, growth, and spread. Circulating HMGB1 level could be a useful and specific marker for evaluating different disease status and predicting prognosis in patients. The label-free quantitative comparison without any standard reference or normalization of the target peptide signal is the simplest Liquid Chromatographic-MS (LC-MS) method for the quantitation of a protein. The aim of the research was the development of an analytical method for the quantitation of HMGB1 by a label-free Mass Spectrometry technique. In this paper are presented the preliminary results: the quantitation of HMGB1 was assessed in a cell lysate sample through the use of the standard concentration curve of purified HMGB1 protein.",

keywords = "HMGB1 quantification, Targeted proteomics, shotgun analysis",

author = "Marcello Manfredi and Simona Martinotti and Mauro Patrone and Sassi, {Maria Paola} and Elia Ranzato and Emilio Marengo",

note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 ; Conference date: 07-05-2015 Through 09-05-2015",

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Manfredi, M , Martinotti, S , Patrone, M, Sassi, MP, Ranzato, E & Marengo, E 2015, Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique. in 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings., 7145260, 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 526-530, 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015, Torino, Italy, 7/05/15. https://doi.org/10.1109/MeMeA.2015.7145260

Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique. / Manfredi, Marcello ; Martinotti, Simona ; Patrone, Mauro et al.
2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. p. 526-530 7145260 (2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Mass spectrometry (MS)-based methods have become popular in recent years for the quantitation of biomolecules, especially peptides and proteins. High Mobility Group Box-1 protein (HMGB1) is a nuclear DNA-binding protein that resides inside the nucleus and can be released to the extracellular space under specific conditions. HMGB1 has been reported as circulating mechanistic indicators of cell death mode in animal models and in clinical studies. There is accumulating evidence that HMGB1 contributes to the pathogenesis of inflammatory and autoimmune diseases. Moreover, HMGB1 has been found to play an important role in tumor development, growth, and spread. Circulating HMGB1 level could be a useful and specific marker for evaluating different disease status and predicting prognosis in patients. The label-free quantitative comparison without any standard reference or normalization of the target peptide signal is the simplest Liquid Chromatographic-MS (LC-MS) method for the quantitation of a protein. The aim of the research was the development of an analytical method for the quantitation of HMGB1 by a label-free Mass Spectrometry technique. In this paper are presented the preliminary results: the quantitation of HMGB1 was assessed in a cell lysate sample through the use of the standard concentration curve of purified HMGB1 protein.

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Manfredi M , Martinotti S , Patrone M, Sassi MP, Ranzato E , Marengo E. Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique. In 2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2015. p. 526-530. 7145260. (2015 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2015 - Proceedings). doi: 10.1109/MeMeA.2015.7145260

Targeted quantitation of HMGB1 protein by label-free Mass Spectrometry technique

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