Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide

Lucia De Rosa; Donatella Diana; Domenica Capasso; Rachele STEFANIA; Rossella Di Stasi; Roberto Fattorusso; Luca Domenico D'Andrea

doi:10.3390/molecules27206982

Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide

Lucia De Rosa, Donatella Diana, Domenica Capasso, Rachele STEFANIA, Rossella Di Stasi, Roberto Fattorusso, Luca Domenico D'Andrea

Department of Science and Technological Innovation

Research output: Contribution to journal › Article › peer-review

Abstract

The N-capping region of an alpha-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.

Original language	English
Journal	Molecules
Volume	27
Issue number	20
DOIs	https://doi.org/10.3390/molecules27206982
Publication status	Published - 2022

Keywords

D-amino acid
N-capping
NMR
peptide conformation
peptide folding
α-helix

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10.3390/molecules27206982

https://hdl.handle.net/11579/185107

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title = "Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide",

abstract = "The N-capping region of an alpha-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.",

keywords = "D-amino acid, N-capping, NMR, peptide conformation, peptide folding, α-helix, D-amino acid, N-capping, NMR, peptide conformation, peptide folding, α-helix",

author = "{De Rosa}, Lucia and Donatella Diana and Domenica Capasso and Rachele STEFANIA and {Di Stasi}, Rossella and Roberto Fattorusso and D'Andrea, {Luca Domenico}",

year = "2022",

doi = "10.3390/molecules27206982",

language = "English",

volume = "27",

journal = "Molecules",

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T1 - Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide

AU - De Rosa, Lucia

AU - Diana, Donatella

AU - Capasso, Domenica

AU - STEFANIA, Rachele

AU - Di Stasi, Rossella

AU - Fattorusso, Roberto

AU - D'Andrea, Luca Domenico

PY - 2022

Y1 - 2022

N2 - The N-capping region of an alpha-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.

AB - The N-capping region of an alpha-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.

KW - D-amino acid

KW - N-capping

KW - NMR

KW - peptide conformation

KW - peptide folding

KW - α-helix

KW - D-amino acid

KW - N-capping

KW - NMR

KW - peptide conformation

KW - peptide folding

KW - α-helix

UR - https://iris.uniupo.it/handle/11579/185107

U2 - 10.3390/molecules27206982

DO - 10.3390/molecules27206982

M3 - Article

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 20

ER -

Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide

Abstract

Keywords

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