Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions

Elena Chiarpotto; Giuseppe Poli; Emanuele Albano; Enrico Gravela; Mario U. Dianzani

doi:10.1016/0014-4800(84)90035-2

Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions

Elena Chiarpotto
, Giuseppe Poli
, Emanuele Albano
, Enrico Gravela
, Mario U. Dianzani

Research output: Contribution to journal › Article › peer-review

Abstract

Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.

Original language	English
Pages (from-to)	191-201
Number of pages	11
Journal	Experimental and Molecular Pathology
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/0014-4800(84)90035-2
Publication status	Published - Oct 1984
Externally published	Yes

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10.1016/0014-4800(84)90035-2

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title = "Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions",

abstract = "Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.",

author = "Elena Chiarpotto and Giuseppe Poli and Emanuele Albano and Enrico Gravela and Dianzani, \{Mario U.\}",

note = "Funding Information: The authors dedicate this publication to Professor Erwin Schauenstein, Head of the Institute of Biochemistry at the Karl-Franzens University in Graz, Austria, on the occasion of his 65th birthday. These studies have been sponsored by the National Foundation for Cancer Research, Bethesda. Maryland, and by the Consiglio Nazionale delle Ricerche, Rome, Italy.",

year = "1984",

month = oct,

doi = "10.1016/0014-4800(84)90035-2",

language = "English",

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T1 - Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions

AU - Chiarpotto, Elena

AU - Poli, Giuseppe

AU - Albano, Emanuele

AU - Gravela, Enrico

AU - Dianzani, Mario U.

N1 - Funding Information: The authors dedicate this publication to Professor Erwin Schauenstein, Head of the Institute of Biochemistry at the Karl-Franzens University in Graz, Austria, on the occasion of his 65th birthday. These studies have been sponsored by the National Foundation for Cancer Research, Bethesda. Maryland, and by the Consiglio Nazionale delle Ricerche, Rome, Italy.

PY - 1984/10

Y1 - 1984/10

N2 - Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.

AB - Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.

UR - https://www.scopus.com/pages/publications/0021711451

U2 - 10.1016/0014-4800(84)90035-2

DO - 10.1016/0014-4800(84)90035-2

M3 - Article

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EP - 201

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

IS - 2

ER -

Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions

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