Studies on fatty liver with isolated hepatocytes. II. The action of carbon tetrachloride on lipid peroxidation, protein, and triglyceride synthesis and secretion

This report describes some effects of poisoning with carbon tetrachloride on hepatocytes in single cell suspension. In isolated liver cells as well as "in vivo" CCl₄ stimulates lipid peroxidation, inhibits both protein synthesis and protein and lipoprotein secretion and induces fat accumulation within the cells. As the action of CCl₄ on lipid peroxidation, our data confirm that its increase induced by CCl₄ depends on the metabolism of this drug by the NADPH-cytochrome P₄₅₀ enzyme system. Furthermore, data reported here suggest that the onset of the CCl₄-induced decrease of lipoprotein secretion is due to a derangement of the secretory pathway.