STR typing of fixed human tissue: Formalin vs. an alcohol-based method

Sarah Gino; Simona Varacalli; Carlo Robino; Carlo Torre

doi:10.1016/S0531-5131(03)01660-1

STR typing of fixed human tissue: Formalin vs. an alcohol-based method

Sarah Gino
, Simona Varacalli
, Carlo Robino
, Carlo Torre

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad®). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.

Original language	English
Pages (from-to)	611-612
Number of pages	2
Journal	International Congress Series
Volume	1261
Issue number	C
DOIs	https://doi.org/10.1016/S0531-5131(03)01660-1
Publication status	Published - 1 Apr 2004
Externally published	Yes

Keywords

Forensic casework
Paternity testing
STR
Tissue fixation

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10.1016/S0531-5131(03)01660-1

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title = "STR typing of fixed human tissue: Formalin vs. an alcohol-based method",

abstract = "In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad{\textregistered}). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.",

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author = "Sarah Gino and Simona Varacalli and Carlo Robino and Carlo Torre",

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AU - Robino, Carlo

AU - Torre, Carlo

PY - 2004/4/1

Y1 - 2004/4/1

N2 - In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad®). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.

AB - In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad®). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.

KW - Forensic casework

KW - Paternity testing

KW - STR

KW - Tissue fixation

UR - https://www.scopus.com/pages/publications/33344459817

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DO - 10.1016/S0531-5131(03)01660-1

M3 - Article

AN - SCOPUS:33344459817

SN - 0531-5131

VL - 1261

SP - 611

EP - 612

JO - International Congress Series

JF - International Congress Series

IS - C

ER -

STR typing of fixed human tissue: Formalin vs. an alcohol-based method

Abstract

Keywords

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