Sorting of cells from different cell cycle phases using surface antigen expression

Filippo Renò; Francesca Luchetti; Marco Vitale; Armando Gregorini; Massimo Valentini; Stefano Papa

doi:10.1007/BF00122159

Sorting of cells from different cell cycle phases using surface antigen expression

Filippo Renò
, Francesca Luchetti
, Marco Vitale
, Armando Gregorini
, Massimo Valentini
, Stefano Papa

Research output: Contribution to journal › Article › peer-review

Abstract

Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G₁ or S-G₂ phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.

Original language	English
Pages (from-to)	93-98
Number of pages	6
Journal	Methods in Cell Science
Volume	18
Issue number	2
DOIs	https://doi.org/10.1007/BF00122159
Publication status	Published - 1996
Externally published	Yes

Keywords

Cd11a
Cell cycle
Cell sorting
Daudi

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10.1007/BF00122159

Cite this

@article{cbc6605ce22f404c9039334c8410f151,

title = "Sorting of cells from different cell cycle phases using surface antigen expression",

abstract = "Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G1 or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.",

keywords = "Cd11a, Cell cycle, Cell sorting, Daudi",

author = "Filippo Ren{\`o} and Francesca Luchetti and Marco Vitale and Armando Gregorini and Massimo Valentini and Stefano Papa",

year = "1996",

doi = "10.1007/BF00122159",

language = "English",

volume = "18",

pages = "93--98",

journal = "Methods in Cell Science",

issn = "1381-5741",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Sorting of cells from different cell cycle phases using surface antigen expression

AU - Renò, Filippo

AU - Luchetti, Francesca

AU - Vitale, Marco

AU - Gregorini, Armando

AU - Valentini, Massimo

AU - Papa, Stefano

PY - 1996

Y1 - 1996

N2 - Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G1 or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.

AB - Detailed procedures are presented for obtaining highly enriched human Daudi cell population in different cell cycle phases. This protocol allows harvest of enriched G1 or S-G2 phase cells based on the recognition of a cell surface marker antigen by anti-CD11a antibody and sorting. Sorted cells are able to cycle and a wave of synchronisation appears. Relative advantages of the method over chemically induced cell synchronisation are discussed.

KW - Cd11a

KW - Cell cycle

KW - Cell sorting

KW - Daudi

UR - https://www.scopus.com/pages/publications/0029797901

U2 - 10.1007/BF00122159

DO - 10.1007/BF00122159

M3 - Article

SN - 1381-5741

VL - 18

SP - 93

EP - 98

JO - Methods in Cell Science

JF - Methods in Cell Science

IS - 2

ER -

Sorting of cells from different cell cycle phases using surface antigen expression

Abstract

Keywords

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