Single-channel current simulation and recording using a photodiode as current generator

D. Bertrand; C. R. Bader; C. Distasi; I. C. Forster

doi:10.1016/0165-0270(89)90121-0

Single-channel current simulation and recording using a photodiode as current generator

D. Bertrand
, C. R. Bader
, C. Distasi
, I. C. Forster

Research output: Contribution to journal › Article › peer-review

Abstract

A device which can generate rectangular currents in the picoampere range is described. The current generator is a photodiode connected to the head stage of a single-channel recording amplifier. The photodiode is activated by a light-emitting diode controlled by a computer or any other current source. The device can transmit signals corresponding to simulated single-channel behaviour. Since the kinetic parameters of the simulation are known, the user can test the data acquisition and analysis system under conditions similar to those prevailing during recording from a biological membrane. This current generator can also be used for the tuning of patch-clamp amplifiers; rectangular currents generated by the photodiode allow the frequency response of the amplifier to be properly adjusted.

Original language	English
Pages (from-to)	233-238
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	26
Issue number	3
DOIs	https://doi.org/10.1016/0165-0270(89)90121-0
Publication status	Published - Jan 1989
Externally published	Yes

Keywords

Model
Simulation
Single channel

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10.1016/0165-0270(89)90121-0

Cite this

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title = "Single-channel current simulation and recording using a photodiode as current generator",

abstract = "A device which can generate rectangular currents in the picoampere range is described. The current generator is a photodiode connected to the head stage of a single-channel recording amplifier. The photodiode is activated by a light-emitting diode controlled by a computer or any other current source. The device can transmit signals corresponding to simulated single-channel behaviour. Since the kinetic parameters of the simulation are known, the user can test the data acquisition and analysis system under conditions similar to those prevailing during recording from a biological membrane. This current generator can also be used for the tuning of patch-clamp amplifiers; rectangular currents generated by the photodiode allow the frequency response of the amplifier to be properly adjusted.",

keywords = "Model, Simulation, Single channel",

author = "D. Bertrand and Bader, \{C. R.\} and C. Distasi and Forster, \{I. C.\}",

note = "Funding Information: This work was supported by Grants 3.594.0.87 and 3.143.0.85 from the Swiss National Science Foundation. We wish to thank Mr. Philippe Cand for his excellent technical assistance and Prof. F. Baumann for his comments on the manuscript.",

year = "1989",

month = jan,

doi = "10.1016/0165-0270(89)90121-0",

language = "English",

volume = "26",

pages = "233--238",

journal = "Journal of Neuroscience Methods",

issn = "0165-0270",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Single-channel current simulation and recording using a photodiode as current generator

AU - Bertrand, D.

AU - Bader, C. R.

AU - Distasi, C.

AU - Forster, I. C.

N1 - Funding Information: This work was supported by Grants 3.594.0.87 and 3.143.0.85 from the Swiss National Science Foundation. We wish to thank Mr. Philippe Cand for his excellent technical assistance and Prof. F. Baumann for his comments on the manuscript.

PY - 1989/1

Y1 - 1989/1

N2 - A device which can generate rectangular currents in the picoampere range is described. The current generator is a photodiode connected to the head stage of a single-channel recording amplifier. The photodiode is activated by a light-emitting diode controlled by a computer or any other current source. The device can transmit signals corresponding to simulated single-channel behaviour. Since the kinetic parameters of the simulation are known, the user can test the data acquisition and analysis system under conditions similar to those prevailing during recording from a biological membrane. This current generator can also be used for the tuning of patch-clamp amplifiers; rectangular currents generated by the photodiode allow the frequency response of the amplifier to be properly adjusted.

AB - A device which can generate rectangular currents in the picoampere range is described. The current generator is a photodiode connected to the head stage of a single-channel recording amplifier. The photodiode is activated by a light-emitting diode controlled by a computer or any other current source. The device can transmit signals corresponding to simulated single-channel behaviour. Since the kinetic parameters of the simulation are known, the user can test the data acquisition and analysis system under conditions similar to those prevailing during recording from a biological membrane. This current generator can also be used for the tuning of patch-clamp amplifiers; rectangular currents generated by the photodiode allow the frequency response of the amplifier to be properly adjusted.

KW - Model

KW - Simulation

KW - Single channel

UR - https://www.scopus.com/pages/publications/0024543149

U2 - 10.1016/0165-0270(89)90121-0

DO - 10.1016/0165-0270(89)90121-0

M3 - Article

C2 - 2918747

AN - SCOPUS:0024543149

SN - 0165-0270

VL - 26

SP - 233

EP - 238

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 3

ER -

Single-channel current simulation and recording using a photodiode as current generator

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Keywords

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