Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology

Pietro Quaglino; Marzia Caproni; Emiliano Antiga; Elena Del Bianco; Simona Osella-Abate; Paola Savoia; Alessandra Frezzolini; Donatella Schena; Angelo Marzano; Walter Volpi; Clara De Simone; Aurora Parodi; Paolo Fabbri; Maria Grazia Bernengo

doi:10.1159/000100880

Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology

Pietro Quaglino, Marzia Caproni, Emiliano Antiga, Elena Del Bianco, Simona Osella-Abate, Paola Savoia, Alessandra Frezzolini, Donatella Schena, Angelo Marzano, Walter Volpi, Clara De Simone, Aurora Parodi, Paolo Fabbri, Maria Grazia Bernengo

Research output: Contribution to journal › Article › peer-review

Abstract

Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

Original language	English
Pages (from-to)	296-304
Number of pages	9
Journal	Dermatology
Volume	214
Issue number	4
DOIs	https://doi.org/10.1159/000100880
Publication status	Published - Apr 2007
Externally published	Yes

Keywords

Erythema multiforme
Stevens-Johnson syndrome/toxic epidermal necrolysis
Thymus- and activation-regulated chemokine

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Quaglino, P., Caproni, M., Antiga, E., Del Bianco, E., Osella-Abate, S., Savoia, P., Frezzolini, A., Schena, D., Marzano, A., Volpi, W., De Simone, C., Parodi, A., Fabbri, P., & Bernengo, M. G. (2007). Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology. Dermatology, 214(4), 296-304. https://doi.org/10.1159/000100880

Quaglino, Pietro ; Caproni, Marzia ; Antiga, Emiliano et al. / Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression : An immunoenzymatic study from the Italian group of immunopathology. In: Dermatology. 2007 ; Vol. 214, No. 4. pp. 296-304.

@article{a16577aa27754a6cbc093a6a13bc6b8f,

title = "Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology",

abstract = "Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.",

keywords = "Erythema multiforme, Stevens-Johnson syndrome/toxic epidermal necrolysis, Thymus- and activation-regulated chemokine",

author = "Pietro Quaglino and Marzia Caproni and Emiliano Antiga and {Del Bianco}, Elena and Simona Osella-Abate and Paola Savoia and Alessandra Frezzolini and Donatella Schena and Angelo Marzano and Walter Volpi and {De Simone}, Clara and Aurora Parodi and Paolo Fabbri and Bernengo, {Maria Grazia}",

year = "2007",

month = apr,

doi = "10.1159/000100880",

language = "English",

volume = "214",

pages = "296--304",

journal = "Dermatology",

issn = "1018-8665",

publisher = "S. Karger AG",

number = "4",

}

Quaglino, P, Caproni, M, Antiga, E, Del Bianco, E, Osella-Abate, S, Savoia, P, Frezzolini, A, Schena, D, Marzano, A, Volpi, W, De Simone, C, Parodi, A, Fabbri, P & Bernengo, MG 2007, 'Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology', Dermatology, vol. 214, no. 4, pp. 296-304. https://doi.org/10.1159/000100880

Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology. / Quaglino, Pietro; Caproni, Marzia; Antiga, Emiliano et al.
In: Dermatology, Vol. 214, No. 4, 04.2007, p. 296-304.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression

T2 - An immunoenzymatic study from the Italian group of immunopathology

AU - Quaglino, Pietro

AU - Caproni, Marzia

AU - Antiga, Emiliano

AU - Del Bianco, Elena

AU - Osella-Abate, Simona

AU - Savoia, Paola

AU - Frezzolini, Alessandra

AU - Schena, Donatella

AU - Marzano, Angelo

AU - Volpi, Walter

AU - De Simone, Clara

AU - Parodi, Aurora

AU - Fabbri, Paolo

AU - Bernengo, Maria Grazia

PY - 2007/4

Y1 - 2007/4

N2 - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

AB - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

KW - Erythema multiforme

KW - Stevens-Johnson syndrome/toxic epidermal necrolysis

KW - Thymus- and activation-regulated chemokine

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U2 - 10.1159/000100880

DO - 10.1159/000100880

M3 - Article

SN - 1018-8665

VL - 214

SP - 296

EP - 304

JO - Dermatology

JF - Dermatology

IS - 4

ER -

Quaglino P, Caproni M, Antiga E, Del Bianco E, Osella-Abate S, Savoia P et al. Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology. Dermatology. 2007 Apr;214(4):296-304. doi: 10.1159/000100880

Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression: An immunoenzymatic study from the Italian group of immunopathology

Abstract

Keywords

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