Scratch wound healing assay

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages225-229
Number of pages5
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2109
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell migration
  • Data analysis
  • Healing assay
  • Scratch wound assay
  • Wound repair

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